DEVELOPMENT AND CHARACTERIZATION OF EST-SSR MARKERS IN THE INVASIVE WEED MIKANIA MICRANTHA (ASTERACEAE)

被引：24

作者：

Yan, Yubin ^{[1
,2
]}

Huang, Yelin ^{[1
,2
]}

Fang, Xiaoting ^{[1
,2
]}

Lu, Lu ^{[1
,2
]}

Zhou, Renchao ^{[1
,2
]}

Ge, Xuejun ^{[3
]}

Shi, Suhua ^{[1
,2
]}

机构：

[1] Sun Yat Sen Univ, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Key Lab Gene Engn Minist Educ, Guangzhou 510275, Guangdong, Peoples R China

[3] Chinese Acad Sci, S China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China

来源：

AMERICAN JOURNAL OF BOTANY | 2011年 / 98卷 / 01期

基金：

中国国家自然科学基金;

关键词：

expressed sequence tag; invasive species; microsatellite; Mikania micrantha;

D O I：

10.3732/ajb.1000387

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Premise of the study : Microsatellite markers were developed for the invasive species Mikania micrantha to investigate its invasion history. Transcriptome sequencing projects offer a potential source for the development of new markers. Methods and Results : Sixteen polymorphic simple sequence repeat (SSR) markers derived from ESTs were identified and screened in 36 samples of M. micrantha. The number of alleles per locus ranged from two to four, with an average of 2.7 alleles per locus. The expected heterozygosity and observed heterozygosity ranged from 0 to 0.7754 and 0 to 0.8333, respectively. Conclusions : These EST-SSR markers may be useful for further investigation of the population genetics of M. micrantha as additional tools to trace its invasion history.

引用

页码：E1 / E3

页数：3

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