Design and implementation of a novel superfusion system for ex vivo characterization of neural tissue by dielectric spectroscopy (DS)

被引：2

作者：

Dobiszewski, K. F. ^{[1
,2
]}

Shaker, M. R. ^{[3
]}

Deek, M. P. ^{[2
,4
,5
]}

Prodan, C. ^{[2
]}

Hill, A. A.

机构：

[1] New Jersey Inst Technol, Mat Sci & Engn Program, Newark, NJ 07102 USA

[2] New Jersey Inst Technol, Dept Phys, Newark, NJ 07102 USA

[3] New Jersey Inst Technol, Dept Biomed Engn, Newark, NJ 07102 USA

[4] New Jersey Inst Technol, Federated Dept Biol Sci, Newark, NJ 07102 USA

[5] Rutgers State Univ, Newark, NJ 07102 USA

来源：

PHYSIOLOGICAL MEASUREMENT | 2011年 / 32卷 / 02期

关键词：

dielectric spectroscopy; superfusion; neural tissue; ex vivo; ELECTROPHYSIOLOGICAL CHANGES; ELECTRICAL-IMPEDANCE; PERFUSION CHAMBER; FREQUENCY;

D O I：

10.1088/0967-3334/32/2/004

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Dielectric spectroscopy is a widely utilized electrophysiological characterization method. The obtained dielectric spectra and derived properties have the potential of providing significant information regarding changes in the physiological state of a biological system. However, since many of the dielectric properties are obtained in vitro from excised tissue far removed from physiological conditions, the value of the information obtained may be diminished. In this paper, we introduce a superfusion system that is designed to produce ex vivo dielectric spectroscopy measurements by providing the living tissue with a continuous and ample supply of nutrients and oxygen while removing metabolites and other waste. This superfusion system provides the convenience of in vitro measurement while concurrently producing results that can be more closely correlated with actual physiological changes in the biological system.

引用

页码：195 / 205

页数：11