Imaging chemistry inside living cells by stimulated Raman scattering microscopy

被引：35
作者：
Lee, Hyeon Jeong [1 ,2 ]
Cheng, Ji-Xin [1 ,2 ,3 ,4 ]
机构：
[1] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA
[2] Purdue Univ, Interdisciplinary Life Sci Program, W Lafayette, IN 47907 USA
[3] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA
[4] Boston Univ, Photon Ctr, Boston, MA 02215 USA
来源：
METHODS | 2017年 / 128卷
关键词：
Chemical imaging; Stimulated Raman scattering microscopy; Vibrational spectroscopy; In vivo imaging; Raman tag; LIVE CELLS; CAENORHABDITIS-ELEGANS; LIPID-METABOLISM; IN-VIVO; CHOLESTEROL; BIOMOLECULES; SENSITIVITY; ORGANELLES; TISSUES; STORAGE;
D O I：
10.1016/j.ymeth.2017.07.020
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Stimulated Raman scattering (SRS) microscopy is a vibrational imaging platform developed to visualize chemical content of a biological sample based on molecular vibrational fingerprints. With high-speed, high-sensitivity, and three-dimensional sectioning capability, SRS microscopy has been used to study chemical distribution, molecular transport, and metabolic conversion in living cells in a label-free manner. Moreover, aided with bio-orthogonal small-volume Raman probes, SRS microscopy allows direct imaging of metabolic activities of small molecules in living cells. (C) 2017 Elsevier Inc. All rights reserved.
引用
收藏
页码：119 / 128
页数：10
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