Detection of American Foulbrood Disease (Paenibacillus larvae) By the PCR and Culture

被引：0

作者：

Kilic, Ayse ^{[1
]}

Simsek, Halil ^{[2
]}

Kalender, Hakan ^{[3
]}

机构：

[1] Firat Univ, Sivrice Vocat High Sch, TR-23119 Elazig, Turkey

[2] Bingol Univ, Vocat Sch Hlth Serv, TR-12000 Bingol, Turkey

[3] Firat Univ, Suleyman Demirel KebanVocat Sch, TR-23740 Elazig, Turkey

来源：

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI | 2010年 / 16卷 / 05期

关键词：

American foulbrood; Culture; PCR; Honey bee; HONEYBEES APIS-MELLIFERA; 16S RIBOSOMAL-RNA; BACILLUS-LARVAE; SUBSP LARVAE; FOUL BROOD; SAMPLES;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

American foulbrood (AFB) caused by Paenibacillus larvae is a bacterial disease in honey bee larvae that is observed worldwide. The aim of this study was to detect P. larvae in honey and beeswax samples from the suspected hives by direct PCR and culture growth. AF6 and AF7, the most sensitive pair of primers, were used to identify the DNA of P. larvae. A total of 100 suspected honey and beeswax samples collected from 25 different enterprises were examined. P. larvae were identified in 7 samples out of 8 by the PCR positive in total. Eight suspected larvae of honeybees were found positive by PCR. A suspected culture-negative sample was found positive through evaluation with PCR. One beeswax sample and seven honey samples were detected as positive for the larvae in the direct PCR detection.

引用

页码：841 / 845

页数：5

共 28 条

[1] Akmaz O., 2001, Pendik Veteriner Mikrobiyoloji Dergisi, V32, P55
[2] Background on American foulbrood in Argentina
Alippi, A
[J]. BEE WORLD, 1997, 78 (02) : 92 - 95
[3] Alippi Adriana M., 1992, Revista Argentina de Microbiologia, V24, P67
[4] Differentiation of Paenibacillus larvae subsp larvae, the cause of American foulbrood of honeybees, by using PCR and restriction fragment analysis of genes encoding 16S rRNA
Alippi, AM
López, AC
Aguilar, OM
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (07) : 3655 - 3660
[5] AYDIN N, 1997, ETLIK VET MIKROB DER, V10, P93
[6] Bailey L., 1981, Honey bee pathology
[7] Development and evaluation of PCR assays for the detection of Paenibacillus larvae in honey samples:: Comparison with isolation and biochemical characterization
Bakonyi, T
Derakhshifar, I
Grabensteiner, E
Nowotny, N
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (03) : 1504 - 1510
[8] BILGEHAN H, 1993, KLIN MIKROBIYOLOJIK, V1
[9] COSKUN SZ, 1994, TURK PARAZIT DERG, V18, P204
[10] DERNAKHSHIFAR I, 1995, BIENENVATER, V116, P464

← 1 2 3 →