Formation of virus-like particles from O-type foot-and-mouth disease virus in insect cells using codon-optimized synthetic genes

被引：25

作者：

Cao, Yimei ^{[1
]}

Sun, Pu ^{[1
]}

Fu, Yuanfang ^{[1
]}

Bai, Xingwen ^{[1
]}

Tian, Feipen ^{[1
]}

Liu, Xiangtao ^{[1
]}

Lu, Zengjun ^{[1
]}

Liu, Zaixin ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol,Key Lab Anim Virol, Natl Foot & Mouth Dis Reference Lab,Minist Agr, Lanzhou 730046, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2010年 / 32卷 / 09期

关键词：

Baculovirus; Codon optimization; Foot-and-mouth disease virus; Virus-like particles; HIGH-LEVEL EXPRESSION; EMPTY CAPSIDS; CLEAVAGE; IMMUNOGENICITY; TRANSLATION; BACULOVIRUS;

D O I：

10.1007/s10529-010-0295-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A recombinant baculovirus was constructed to simultaneously express codon-optimized virus-like particles (VLP), A VP1-2A-VP3 and VP0 of serotype O foot-and-mouth disease virus (FMDV), from individual promoters. The target proteins were expressed in insect cells at high level, as shown by indirect sandwich ELISA; and the expressed VP1-2A-VP3 could autocatalytically be cleaved into the individual proteins, VP1-2A and VP3, as shown by Western-blot analyses. In addition, in the insect cells, the structural proteins, VP0, VP3 and VP1-2A, self-assembled into virus-like particles resembling the authentic FMDV particles. This information should prove useful for the development of more efficient VLP assembly using shorter genes.

引用

页码：1223 / 1229

页数：7

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