Hydrogen peroxide agarose gels for electrophoretic analysis of RNA

被引：4

作者：

Pandey, Renu ^{[1
]}

Saluja, Daman ^{[1
]}

机构：

[1] Univ Delhi, Med Biotechnol Lab, Dr BR Ambedkar Ctr Biomed Res, Delhi 110007, India

来源：

ANALYTICAL BIOCHEMISTRY | 2017年 / 534卷

关键词：

RNAse A; Hydrogen peroxide; Bleach; Electrophoresis; Nuclease; RIBONUCLEASE-A; VIRUS RNASE; H DOMAIN; SUPERFAMILY;

D O I：

10.1016/j.ab.2017.07.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Efficient electrophoretic separation of isolated total RNA utilizes chemicals and agents to aid in nuclease free environment. However cost, extensive pre-run processing protocols as well as toxic byproducts limit the usage of such protocols. Moreover, these treatments affect the overall electrophoretic results by altering the conductivity of the running buffer and weaken the gel strength. We here provide a protocol for RNA visualization that obviates these shortcomings by preparation of agarose gel with hydrogen peroxide using the regular TAE buffer. The simple, inexpensive protocol exhibits superior results in a horizontal agarose gel electrophoresis. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：24 / 27

页数：4

共 16 条

[1] Bleach gel: A simple agarose gel for analyzing RNA quality
Aranda, Patrick S.
LaJoie, Dollie M.
Jorcyk, Cheryl L.
[J]. ELECTROPHORESIS, 2012, 33 (02) : 366 - 369
[2] Stability and folding of amphibian ribonuclease A superfamily members in comparison with mammalian homologues
Arnold, Ulrich
[J]. FEBS JOURNAL, 2014, 281 (16) : 3559 - 3575
[3] A Review of Ribonuclease 7's Structure, Regulation, and Contributions to Host Defense
Becknell, Brian
Spencer, John David
[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2016, 17 (03):
[4] The RNase a superfamily: Generation of diversity and innate host defense
Dyer, Kimberly D.
Rosenberg, Helene F.
[J]. MOLECULAR DIVERSITY, 2006, 10 (04) : 585 - 597
[5] A SIMPLE PROCEDURE FOR GEL-ELECTROPHORESIS AND NORTHERN BLOTTING OF RNA
GODA, SK
MINTON, NP
[J]. NUCLEIC ACIDS RESEARCH, 1995, 23 (16) : 3357 - 3358
[6] RIBONUCLEASE IN BOVINE MILK
IBUKI, F
MORI, T
MATSUSHITA, S
HATA, T
[J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1965, 29 (07): : 635 - +
[7] Hydrogen peroxide-induced structural alterations of RNase A
Lasch, P
Petras, T
Ullrich, O
Backmann, J
Naumann, D
Grune, T
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (12) : 9492 - 9502
[8] Crystal structure of the Moloney murine leukemia virus RNase H domain
Lim, David
Gregorio, G. Glenn
Bingman, Craig
Martinez-Hackert, Erik
Hendrickson, Wayne A.
Goff, Stephen P.
[J]. JOURNAL OF VIROLOGY, 2006, 80 (17) : 8379 - 8389
[9] Denaturing RNA electrophoresis in TAE agarose gels
Masek, T
Vopalensky, V
Suchomelova, P
Pospisek, M
[J]. ANALYTICAL BIOCHEMISTRY, 2005, 336 (01) : 46 - 50
[10] Irreversible Thermoinactivation of Ribonuclease-A by Soft-Hydrothermal Processing
Miyamoto, Toru
Okano, Shinya
Kasai, Noriyuki
[J]. BIOTECHNOLOGY PROGRESS, 2009, 25 (06) : 1678 - 1685

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