Epicatechin Gallate Suppresses Oxidative Stress-Induced MUC5AC Overexpression by Interaction with Epidermal Growth Factor Receptor

被引:20
作者
Kim, Hyun Jik [2 ]
Ryu, Ji-Hwan [5 ]
Kim, Chang-Hoon [1 ,3 ]
Lim, Jun Wan [1 ]
Moon, Uk Yeol [3 ]
Lee, Gyu Ho [2 ]
Lee, Jeung-Gweon [1 ]
Baek, Seung Joon [6 ]
Yoon, Joo-Heon [1 ,3 ,4 ,5 ]
机构
[1] Yonsei Univ, Coll Med, Dept Otorhinolaryngol, Seoul 120752, South Korea
[2] Chung Ang Univ, Coll Med, Dept Otolaryngol Head & Neck Surg, Seoul 156756, South Korea
[3] Yonsei Univ, Coll Med, Airway Mucus Inst, Seoul 120752, South Korea
[4] Yonsei Univ, Coll Med, Brain Korea Project Med Sci 21, Seoul 120752, South Korea
[5] Yonsei Univ, Coll Med, Res Ctr Human Nat Def Syst, Seoul 120752, South Korea
[6] Univ Tennessee, Coll Vet Med, Dept Pathobiol, Knoxville, TN 37901 USA
关键词
epicatechin gallate; epidermal growth factor receptor; mucus hypersecretion; oxidative stress; CELL-CYCLE ARREST; EPITHELIAL-CELLS; GREEN TEA; HYDROGEN-PEROXIDE; MUCIN PRODUCTION; RETINOIC ACID; INDUCTION; APOPTOSIS; (-)-EPIGALLOCATECHIN-3-GALLATE; DIFFERENTIATION;
D O I
10.1165/rcmb.2009-0205OC
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The goal of this study was to investigate the effect of epicatechin gallate (ECG), a component of green tea polyphenols, on the signal pathway for oxidative stress-induced intracellular reactive oxygen species (ROS) generation and MUC5AC overexpression in normal human nasal epithelial (NHNE) cells. Passage-2 NHNE cells were used, and ECG was administered before stimulation with exogenous hydrogen peroxide (H2O2). MUC5AC gene and protein levels were measured by real-time PCR and dot blot analysis. Western blot analysis and immunocytofluorescence study were performed for detecting the activity of epidermal growth factor receptor (EGFR). Exogenous H2O2 increases intracellular ROS generation, leading to the overexpression of MUC5AC. The phosphorylation and internalization of EGFR were associated with this ROS generation. ECG decreased the phosphorylation and internalization of EGFR at the cell surface of NHNE cells, resulting in the attenuation of exogenous H2O2-induced intracellular ROS generation and MUC5AC overexpression. ECG may be a therapeutic material against oxidative stress-induced ROS generation and mucus hypersecretion in airways.
引用
收藏
页码:349 / 357
页数:9
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