Complete nucleotide sequence of a severe isolate of cucumber vein yellowing virus from Jordan

被引:5
|
作者
Galipienso, L. [1 ]
Rubio, L. [2 ]
Aramburu, J. [1 ]
Velasco, L. [3 ]
Janssen, D. [4 ]
机构
[1] Inst Invest & Tecnol Agroalimentaria IRTA, Dept Patol Vegetal, Barcelona 08348, Spain
[2] IVIA, Valencia 46113, Spain
[3] Ctr Churriana, Inst Andaluz Invest & Formac Agr & Pesquera IFAPA, Malaga 29140, Spain
[4] Ctr Mojonera, Inst Andaluz Invest & Formac Agr & Pesquera IFAPA, Almeria 04745, Spain
关键词
PROTEASE; GENOME; MEMBER; ACID;
D O I
10.1007/s00705-012-1264-4
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The complete genome of a severe isolate of Cucumber vein yellowing virus (CVYV) from Jordan was sequenced. Comparison with the genome of a Spanish CVYV isolate inducing very mild symptoms in cucumber cultivars revealed a nucleotide identity of 94% for the complete genome and an amino acid identity of 96% for the coding region. Comparison of synonymous and non-synonymous substitutions suggested a negative selection at amino acid and nucleotide levels with different degrees depending on the different coding regions. Finally, specific amino acid changes in the zinc finger domain of P1b and in the P1-P3 proteolytic site were found which could be involved in the virulence of CVYV.
引用
收藏
页码:1189 / 1192
页数:4
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