The narQP genes for a two-component regulatory system from the deep-sea bacterium Shewanella violacea DSS12

被引：4

作者：

Tamegai, Hideyuki ^{[1
]}

Chikuma, Sayaka ^{[1
]}

Ishi, Masami ^{[1
]}

Nakasone, Kaoru ^{[2
]}

Kato, Chiaki ^{[3
]}

机构：

[1] Nihon Univ, Dept Chem, Coll Humanities & Sci, Setagaya Ku, Tokyo 1568550, Japan

[2] Kinki Univ, Dept Biotechnol & Chem, Sch Engn, Hiroshima, Japan

[3] Japan Agcy Marine Earth Sci & Technol, Extremobiosphere Res Ctr, Yokosuka, Kanagawa 2370061, Japan

来源：

DNA SEQUENCE | 2008年 / 19卷 / 03期

关键词：

Shewanella violacea; narP; respiratory system; pressure-regulation of gene expression; two-component reguiatory system;

D O I：

10.1080/10425170701605856

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Shewanella violacea DSS12 is facultative piezophile isolated from the deep-sea. The expression of cydDC genes (required for d-type cytochrome maturation) of the organism is regulated by hydrostatic pressure. In this study, we analyzed the nucleotide sequence upstream of cydDC in detail and found that there are putative binding sites for the NarL protein which is part of a two-component regulatory system also containing the sensor protein NarX. Furthermore, we identified the narQP genes (homologues of narXL) from S. violacea DSS12 and demonstrated the heterologous expression of narP in Escherichia coli. These results will be helpful in examining pressure regulation of gene expression in S. violacea at the molecular level.

引用

页码：308 / 312

页数：5

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