Expression of P-glycoprotein and multidrug resistance-associated protein on human brain-microvascular endothelial cells with electromagnetic stimulation

被引：17

作者：

Kuo, Yung-Chih ^{[1
]}

Lu, Chin-Hsun ^{[1
]}

机构：

[1] Natl Chung Cheng Univ, Dept Chem Engn, Chiayi 62102, Taiwan

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2012年 / 91卷

关键词：

Electromagnetic field; P-glycoprotein; Multidrug resistance-associated protein; Endothelium; Saquinavir; Azidothymidine; DRUG-RESISTANCE; BARRIER; TRANSPORT; SAQUINAVIR; INHIBITORS; HOMOLOGS; SYSTEM;

D O I：

10.1016/j.colsurfb.2011.10.035

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

This study investigates the effect of electromagnetic field (EMF) on the expression of P-glycoprotein (P-gp) and multidrug resistance-associated protein (MRP) on human brain-microvascular endothelial cells (HBMECs). A monolayer of HBMECs was cocultured with human astrocytes for developing apical/basolateral membrane properties. The results revealed that an increase in the frequency of an EMF enhanced the endocytotic uptake of calcein-AM. Probenecid demonstrated a stronger inhibition to the efflux pumps than verapamil under the exposure to an EMF. The expressed quantities of P-gp and MRP1 after transcytosis of saquinavir were higher than those of azidothymidine, suggesting that the former induced a stronger drug resistance than the latter. In addition, an EMF with 750 Hz could effectively inhibit the expression of P-gp and MRP1. An appropriate exposure to an EMF can be efficient to deliver antiretroviral drugs into the brain via reducing the expressed efflux transporters on HBMECs. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：57 / 62

页数：6

共 29 条

[1] The human multidrug resistance protein 4 (MRP4, ABCC4):: Functional analysis of a highly polymorphic gene [J].

Abla, Nada ;

Chinn, Leslie W. ;

Nakamura, Tsutomu ;

Liu, Li ;

Huang, Conrad C. ;

Johns, Susan J. ;

Kawamoto, Michiko ;

Stryke, Doug ;

Taylor, Travis R. ;

Ferrin, Thomas E. ;

Giacomini, Kathleen M. ;

Kroetz, Deanna L. .

JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2008, 325 (03) :859-868

[2] REVERSIBLE MICROWAVE EFFECTS ON THE BLOOD-BRAIN-BARRIER [J].

ALBERT, EN ;

KERNS, JM .

BRAIN RESEARCH, 1981, 230 (1-2) :153-164

[3]

Allen JD, 2002, MOL CANCER THER, V1, P427

[4] Quantitative assessment of HIV-1 protease inhibitor interactions with drug efflux transporters in the blood-brain barrier [J].

Bachmeier, CJ ;

Spitzenberger, TJ ;

Elmquist, WF ;

Miller, DW .

PHARMACEUTICAL RESEARCH, 2005, 22 (08) :1259-1268

[5]

Bauer B, 2005, EXP BIOL MED, V230, P118

[6] Multidrug resistance-associated proteins: Expression and function in the central nervous system [J].

Dallas, Shannon ;

Miller, David S. ;

Bendayan, Reina .

PHARMACOLOGICAL REVIEWS, 2006, 58 (02) :140-161

[7] A multidrug resistance transporter from human MCF-7 breast cancer cells [J].

Doyle, LA ;

Yang, WD ;

Abruzzo, LV ;

Krogmann, T ;

Gao, YM ;

Rishi, AK ;

Ross, DD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (26) :15665-15670

[8] GF120918, a P-glycoprotein modulator, increases the concentration of unbound amprenavir in the central nervous system in rats [J].

Edwards, JE ;

Brouwer, KR ;

McNamara, PJ .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (07) :2284-2286

[9] Characterisation of the brain multidrug resistance protein (BMDP/ABCG2/BCRP) expressed at the blood-brain barrier [J].

Eisenblätter, T ;

Hüwel, S ;

Galla, HJ .

BRAIN RESEARCH, 2003, 971 (02) :221-231

[10] Transport of HIV protease inhibitors through the blood-brain barrier and interactions with the efflux proteins, P-glycoprotein and multidrug resistance proteins [J].

Gimenez, F ;

Fernandez, C ;

Mabondzo, A .

JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES, 2004, 36 (02) :649-658

← 1 2 3 →