Critical roles of mRNA m6A modification and YTHDC2 expression for meiotic initiation and progression in female germ cells

被引：17
作者：
Zeng, Ming [1 ]
Dai, Xin [1 ]
Liang, Zhibing [1 ]
Sun, Ruliang [2 ]
Huang, Sui [2 ]
Luo, Liangping [3 ]
Li, Zhongxiang [1 ]
机构：
[1] Jinan Univ, Baoan Womens & Childrens Hosp, Med Res Inst, 56 Yulv Rd, Shenzhen 518133, Peoples R China
[2] Jinan Univ, Baoan Womens & Childrens Hosp, Dept Pathol, Shenzhen 518133, Peoples R China
[3] Jinan Univ, Med Imaging Ctr, Affiliated Hosp 1, 613 West Huangpu Rd, Guangzhou 510630, Peoples R China
来源：
GENE | 2020年 / 753卷
基金：
中国博士后科学基金;
关键词：
N-6-methyladenosine (m(6)A); YTHDC2; Female germ cells; Meiotic initiation; MEIOSIS; OOGENESIS;
D O I：
10.1016/j.gene.2020.144810
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Meiotic entry and progression require dynamic regulation of germline gene expression. m(6)A on mRNAs and recognition by YTHDC2 has been known as post-transcriptional regulatory complex, but the roles of this regulator remain unclear for meiotic initiation and progression in female germ cells (FGCs). This study showed that m(6)A modification occurred mainly in FGCs rather than ovarian somatic cells (SOMAS), and m(6)A levels in FGCs increased significantly with meiotic initiation. m(6)A inhibition suppressed expression of the meiotic markers and affected the percent of FGCs at zygotene, pachytene and diplotene stage respectively. YTHDC2 expression also increased in the same pattern with m(6)A. Ythdc2 knockdown decreased the percent of STRA8-positive FGCs and altered the percent of FGCs at zygotene and pachytene stage respectively. Taken together, these results suggest that mRNA m(6)A modification and YTHDC2 expression are essential for meiotic initiation and progression in FGCs.
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页数：6
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