ROS-Responsive Chitosan Coated Magnetic Iron Oxide Nanoparticles as Potential Vehicles for Targeted Drug Delivery in Cancer Therapy

被引:41
作者
Ayyanaar, Srinivasan [1 ]
Balachandran, Chandrasekar [2 ]
Bhaskar, Rangaswamy Chinnabba [3 ]
Kesavan, Mookkandi Palsamy [4 ]
Aoki, Shin [2 ,5 ]
Raja, Ramachandran Palpandi [6 ]
Rajesh, Jegathalaprathaban [7 ]
Webster, Thomas J. [8 ]
Rajagopal, Gurusamy [1 ]
机构
[1] Chikkanna Govt Arts Coll, PG & Res Dept Chem, Tiruppur 641602, Tamil Nadu, India
[2] Tokyo Univ Sci, Fac Pharmaceut Sci, Noda, Chiba 2788510, Japan
[3] Vellore Inst Technol, Sch Adv Sci, Dept Chem, Vellore 632014, Tamil Nadu, India
[4] Hajee Karutha Rowther Howdia Coll, Dept Chem, Uthamapalayam 625533, Tamil Nadu, India
[5] Tokyo Univ Sci, Res Inst Sci & Technol, Noda, Chiba 278850, Japan
[6] Anna Univ, Ctr Biotechnol, Chennai 600025, Tamil Nadu, India
[7] Mohamed Sathak Engn Coll, Chem Res Ctr, Kilakarai 623806, Tamil Nadu, India
[8] Northeastern Univ, Dept Chem Engn, Boston, MA 02115 USA
关键词
magnetic iron oxide nanoparticles; oleic acid; chitosan; 5-fluorouracil; cytotoxicity; targeted drug delivery; MICROSPHERES; 5-FLUOROURACIL; CURCUMIN; NANOTHERANOSTICS; MULTILAYERS; FABRICATION; RESISTANCE; PARTICLES; EXPOSURE; CARRIERS;
D O I
10.2147/IJN.S249240
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Background and Objective: Cancer cells accumulate high concentrations of reactive oxygen species as a result of their faster and uninhibited metabolic activity. Cancer chemotherapeutic agents release an excess of severe adverse reactions as a result of targeting normal cells. This demands an improvement in targeted drug-delivery systems to selectively discharge anticancer drugs in the vicinity of such highly metabolically and mitotically active cells. Materials and Methods: Here, magnetic nanoparticles were synthesized by a traditional co-precipitation technique. Fe3O4@OA-CS-5-FLU-NPs were synthesized by an easy and rapid in situ loading method. The proposed Fe3O4@OA-CS-5-FLU-NPs were productively prepared as well as characterized by various spectroscopic and microscopic studies. Results: The targeted drug release profile of the Fe3O4@OA-CS-5-FLU-NPs was studied in the presence of ROS including H2O2 and pH induction. The released product, Fe3O4@OA-CS-5-FLU-NP, exhibited desirable levels of cytotoxicity and demonstrated morphological changes and inhibition of colony formation for A549 and HeLa S3 cancer cells. The IC50 values at 24 hours were 12.9 and 23 mu g/mL, respectively. Conclusion: In summary, results from the MTT assay, fluorescence staining as well as colony formation assays, revealed that the Fe3O4@OA-CS-5-FLU-NPs were active and safe for anticancer biomedical applications. In summary, the present investigation provides a powerful nanostructured based system for improved cancer theranostics that should be further studied.
引用
收藏
页码:3333 / 3346
页数:14
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