Establishment of stable GH3 cell line expressing enhanced yellow fluorescein protein-growth hormone fusion protein

被引：9

作者：

Matsuno, A

Mizutani, A

Itoh, J

Takekoshi, S

Nagashima, T

Okinaga, H

Takano, K

Osamura, RY

机构：

[1] Teikyo Univ, Dept Neurosurg, Ichihara Hosp, Ichihara, Chiba 2990111, Japan

[2] Tokai Univ, Sch Med, Dept Pathol, Isehara, Kanagawa, Japan

[3] Tokai Univ, Sch Med, Teaching & Res Support Ctr, Isehara, Kanagawa, Japan

[4] Tokyo Univ Hosp, Dept Internal Med, Div Endocrinol, Bunkyo Ku, Tokyo, Japan

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2005年 / 53卷 / 09期

关键词：

EYFP; GH; GH3; cell;

D O I：

10.1369/jhc.5B6708.2005

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

To investigate, in real time, the transport and secretion of pituitary hormone, we have developed an experimental pituitary cell line, GH3 cell, which has secretory granules of growth hormone (GH) linked to enhanced yellow fluorescein protein (EYFP). This stable GH(3) cell secretes secretory granules of GH linked to EYFP on stimulation by Ca2+ influx or Ca-2 release from storage. This GH3 cell will be useful for the real-time visualization of the intracellular transport and secretion of GH.

引用

页码：1177 / 1180

页数：4

共 4 条

[1] A secreted fluorescent reporter targeted to pituitary growth hormone cells in transgenic mice [J].