Tracking of phenol degrading genotype

被引：20

作者：

Kapley, A ^{[1
]}

Purohit, HJ ^{[1
]}

机构：

[1] Natl Environm Engn Res Inst, Nagpur 440020, Maharashtra, India

来源：

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH | 2001年 / 8卷 / 02期

关键词：

bioremediation; dmp; industrial effluent; letters; PCR; phe; phenol degradation; pseudomonas; refinery wastewaters; tracking; wastewaters;

D O I：

10.1007/BF02987299

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A simple and rapid protocol has been developed for monitoring of the phenol degrading population in the environmental samples. PCR protocol uses the total DNA prepared from the samples as a template in the amplification reaction. Primers have been designed from the sequence data for Dmp and Phe operon encoding multi-component and single component phenol oxidizing system, respectively. The phenol degrading genotype in various samples have been demonstrated using the developed PCR protocol.

引用

页码：89 / 90

页数：2

共 7 条

[1]

ATLAS RM, 1977, CRIT REV MICROBIOL, V15, P371

[2] RELATIONSHIP BETWEEN HYDROCARBONS AND BACTERIAL-ACTIVITY IN MEDITERRANEAN SEDIMENTS .2. HYDROCARBON DEGRADING ACTIVITY OF BACTERIA FROM SEDIMENTS [J].

AZOULAY, E ;

COLIN, M ;

DUBREUIL, J ;

DOU, H ;

MILLE, G ;

GIUSTI, G .

MARINE ENVIRONMENTAL RESEARCH, 1983, 9 (01) :19-36

[3] Bacterial consortia for crude oil spill remediation [J].

Chhatre, S ;

Purohit, H ;

Shanker, R ;

Khanna, P .

WATER SCIENCE AND TECHNOLOGY, 1996, 34 (10) :187-193

[4] Osmotolerance and hydrocarbon degradation by a genetically engineered microbial consortium [J].

Kapley, A ;

Purohit, HJ ;

Chhatre, S ;

Shanker, R ;

Chakrabarti, T ;

Khanna, P .

BIORESOURCE TECHNOLOGY, 1999, 67 (03) :241-245

[5] SEQUENCE OF THE GENE (PHEA) ENCODING PHENOL MONOOXYGENASE FROM PSEUDOMONAS SP-EST1001 - EXPRESSION IN ESCHERICHIA-COLI AND PSEUDOMONAS-PUTIDA [J].

NURK, A ;

KASAK, L ;

KIVISAAR, M .

GENE, 1991, 102 (01) :13-18

[6]

Sambrook J., 1989, MOL CLONING, V2, P1

[7] SENSING OF AROMATIC-COMPOUNDS BY THE DMPR TRANSCRIPTIONAL ACTIVATOR OF PHENOL-CATABOLIZING PSEUDOMONAS SP STRAIN CF600 [J].

SHINGLER, V ;

MOORE, T .

JOURNAL OF BACTERIOLOGY, 1994, 176 (06) :1555-1560

← 1 →