Genetic characterisation of drug resistance and clonal dynamics of Acinetobacter baumannii in a hospital setting in Mexico

被引:43
作者
Bocanegra-Ibarias, Paola [1 ]
Pena-Lopez, Cynthia [2 ]
Camacho-Ortiz, Adrian [3 ]
Llaca-Diaz, Jorge [4 ]
Silva-Sanchez, Jesus [5 ]
Barrios, Humberto [5 ]
Garza-Ramos, Ulises [5 ]
Rodriguez-Flores, Adrian Marcelo [2 ]
Garza-Gonzalez, Elvira [1 ,4 ]
机构
[1] Hosp Univ Dr Jose Eleuterio Gonzalez, Serv Gastroenterol, Monterrey, Nuevo Leon, Mexico
[2] Hosp Univ Dr Jose Eleuterio Gonzalez, Dept Med Interna, Monterrey, Nuevo Leon, Mexico
[3] Hosp Univ Dr Jose Eleuterio Gonzalez, Serv Infectol Coordinac Epidemiol Hosp, Monterrey, Nuevo Leon, Mexico
[4] Univ Autonoma Nuevo Leon, Hosp Univ Dr Jose Eleuterio Gonzalez, Dept Patol Clin, San Nicolas De Los Garza, Nuevo Leon, Mexico
[5] Inst Nacl Salud Publ, Dept Diagnost Epidemiol, Cuernavaca, Morelos, Mexico
关键词
Acinetobacter; PFGE; Carbapenemases; OXA-58; OXA-72; Biofilm; CRITICALLY-ILL PATIENTS; MULTIPLEX PCR; CARBAPENEMASES; STRAINS;
D O I
10.1016/j.ijantimicag.2014.10.022
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The aim of this study was to determine the clinical characteristics, molecular epidemiology and biofilm production of Acinetobacter baumannii clinical isolates obtained from a tertiary-care hospital in Mexico. Clinical isolates of A. baumannii (n = 152) isolated from 2007 to 2012 were included. Clonal diversity was analysed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Antimicrobial susceptibility was determined using the broth microdilution method. IMP, VIM, NDM and OXA-type genes were screened by PCR. Biofilm production was analysed using the crystal violet method. Mortality attributable to A. baumannii infection was 14.5%. Fifty-four clones were detected, of which five predominated. MLST results showed three new sequence types and two reported sequence types. More than 86% of the isolates were resistant to ciprofloxacin, ceftazidime and cefotaxime. Furthermore, 50.7% and 35.5% of the isolates were resistant to imipenem and meropenem, respectively. Of the isolates evaluated, 28.3% and 25.7% were positive for the bla(OXA-58) and bla(OXA-72) genes, respectively. Biofilm production was associated with resistance to imipenem (P = 0.002). (C) 2014 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
引用
收藏
页码:309 / 313
页数:5
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