TEMPERATURE DEPENDENT GROWTH CHARACTERISTICS AND A PREDICTIVE MATHEMATICAL MODEL OF BACILLUS CEREUS IN WET NOODLES

We examined the growth characteristics and developed a mathematical model to predict the growth kinetics of Bacillus cereus in fresh wet noodles. Noodles were incubated with B. cereus at 5, 10, 20 and 30C. The primary model that we developed to obtain the specific growth rate (SGR) and lag time (LT) fit well (r(2) = 0.9621-0.9977) with the Gompertz equation. The SGR of B. cereus increased with increasing temperature. A secondary polynomial model was obtained by SAS regression analysis and calculated as: SGR = -0.0128 + 0.00229(star)T + 0.0003(star)T(2). The appropriateness of this secondary polynomial model was verified by the mean square error (MSE = 0.0001 for SGR model), bias factor (B-f = 0.9012 for SGR model), accuracy factor (A(f) = 1.3333 for SGR model) and coefficient of determination (r(2) = 0.9955 for SGR). This secondary polynomial model is therefore in good agreement with the validation calculations and may be used as a practical predictive model for the growth of B. cereus in wet noodles as a function of storage temperature.