Vaccination with recombinant L7/L12-truncated Omp31 protein induces protection against Brucella infection in BALB/c mice

被引：30

作者：

Golshani, Maryam ^{[1
]}

Rafati, Sima ^{[2
]}

Dashti, Arnir ^{[1
]}

Gholami, Elham ^{[2
]}

Siadat, Seyed Davar ^{[3
]}

Oloomi, Mana ^{[1
]}

Jafari, Anis ^{[1
]}

Bouzari, Saeid ^{[1
]}

机构：

[1] Pasteur Inst Iran, Dept Mol Biol, Tehran, Iran

[2] Pasteur Inst Iran, Dept Mol Immunol & Vaccine Res, Tehran, Iran

[3] Pasteur Inst Iran, Dept Bacteriol, Tehran, Iran

来源：

MOLECULAR IMMUNOLOGY | 2015年 / 65卷 / 02期

关键词：

Brucella melitensis; Brucella abortus; Fusion protein; Human vaccine; L7/L12; Truncated Omp31; CPG OLIGODEOXYNUCLEOTIDES ACT; MELITENSIS; ADJUVANTS; ABORTUS; L7/L12; IMMUNIZATION; VACCINES; IMMUNITY; OVIS;

D O I：

10.1016/j.molimm.2015.01.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Brucellosis is the most common bacterial zoonotic disease worldwide and no vaccine is available for the prevention of human brucellosis. In humans, brucellosis is mostly caused by Brucella melitensis and Brucella abortus. The Outer membrane protein 31 (Omp31) and L7/L12 are immunodominant and protective antigens conserved in human Brucella pathogens. In the present study, we evaluated the humoral and cellular immune responses induced by a fusion protein designed based on the Truncated form of Omp31 (TOmp31) and L7-L12 antigens. Vaccination of BALB/c mice with the recombinant fusion protein (rL7/L12-TOmp31) provided the significant protection level against B. melitensis and B. abortus challenge. Moreover, rL7/L12-TOmp31 elicited a strong specific IgG response (higher IgG2a titers) and significant IFN-gamma/IL2 production and T-cell proliferation was also observed. The T helper1 (Th1) oriented response persisted for 12 weeks after the first immunization. The rL7/L12-TOmp31 could be a new potential antigen candidate for the development of a subunit vaccine against B. melitensis and B. abortus. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：287 / 292

页数：6

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