Targeted high-throughput sequencing of tagged nucleic acid samples

被引：139

作者：

Meyer, Matthias ^{[1
]}

Stenzel, Udo ^{[1
]}

Myles, Sean ^{[1
]}

Pruefer, Kay ^{[1
]}

Hofreiter, Michael ^{[1
]}

机构：

[1] Max Planck Inst Evolutionary Anthropol, D-04103 Leipzig, Germany

来源：

NUCLEIC ACIDS RESEARCH | 2007年 / 35卷 / 15期

关键词：

D O I：

10.1093/nar/gkm566

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High-throughput 454 DNA sequencing technology allows much faster and more cost-effective sequencing than traditional Sanger sequencing. However, the technology imposes inherent limitations on the number of samples that can be processed in parallel. Here we introduce parallel tagged sequencing (PTS), a simple, inexpensive and flexible barcoding technique that can be used for parallel sequencing any number and type of double-stranded nucleic acid samples. We demonstrate that PTS is particularly powerful for sequencing contiguous DNA fragments such as mtDNA genomes: in theory as many as 250 mammalian mtDNA genomes can be sequenced in a single GS FLX run. PTS dramatically increases the sequencing throughput of samples in parallel and thus fully mobilizes the resources of the 454 technology for targeted sequencing.

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