Epigenetic Alteration of the Wnt Inhibitory Factor-1 Promoter Is Common and Occurs in Advanced Stage of Tunisian Nasopharyngeal Carcinoma

被引：23

作者：

Fendri, Ali ^{[1
]}

Khabir, Abdelmajid ^{[2
]}

Hadri-Guiga, Boutheina ^{[1
]}

Sellami-Boudawara, Tahia ^{[2
]}

Daoud, Jamel ^{[2
]}

Frikha, Mounir ^{[2
]}

Ghorbel, Abdelmonem ^{[2
]}

Gargouri, Ali ^{[1
]}

Mokdad-Gargouri, Raja ^{[1
]}

机构：

[1] Ctr Biotechnol Sfax, Unit Rech Genet Canc & Prod Prot Therapeut, Sfax 3038, Tunisia

[2] Ctr Hosp Univ Habib Bourguiba, Sfax, Tunisia

来源：

CANCER INVESTIGATION | 2010年 / 28卷 / 09期

关键词：

Nasopharyngeal carcinoma; WIF-1; Wnt-5a; Methylation-specific PCR; Reverse PCR; TUMOR-SUPPRESSOR; UP-REGULATION; BETA-CATENIN; LUNG-CANCER; EXPRESSION; GENE; ACTIVATION; PATHWAY; INACTIVATION; BREAST;

D O I：

10.3109/07357907.2010.494324

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Activation of the wingless-type (Wnt) signaling pathway is common in cancers. The Wnt inhibitory factor-1 (WIF-1) is a secreted antagonist that acts by binding to Wnt ligands. We examined by methylation-specific PCR (MSP), whether WIF-1 is inactivated in 68 nasopharyngeal carcinomas (NPC), and 10 normal mucosa. We showed that the WIF-1 promoter was methylated in 89.7% of tumors, whereas all normal mucosa were unmethylated. The WIF-1 methylation was associated with the tumor, node, and metastasis (TNM) (p = .003) and the age (p = .014). The Wnt-5a mRNA was higher in tumors and correlated with TNM (p = .012). The methylation of WIF-1 contributes to the activation of the Wnt pathway in NPC.

引用

页码：896 / 903

页数：8

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