Unregulated international flow of foods poses a danger to human health, as it may be contaminated with pathogens. Recent studies have investigated neglected routes of pathogen transmission and reported the occurrence of Listeria monocytogenes in food illegally imported into the European Union (EU), either confiscated at four international airports or sold illegally on the Romanian black market. In this study we investigated the genotype diversity and the amino acid sequence variability of three main virulence factors of 57 L. monocytogenes isolates. These isolates were derived from 1474 food samples illegally imported into the EU and originated from 17 different countries. Multilocus sequence typing revealed 16 different sequence types (STs) indicating moderate genotype diversity. The most prevalent STs were ST2, ST9, and ST121. The pulsed-field gel electrophoresis (PFGE) analysis resulted in 34 unique pulsotypes. PFGE types assigned to the most prevalent STs (ST2, ST9, and ST121) were highly related in their genetic fingerprint. Internalin A (InlA) was present in 20 variants, including six truncated InlA variants, all harbored by isolates of ST9 and ST121. We detected eight ST-specific listeriolysin O (LLO) variants, and among them, one truncated form. The actin-assembly-inducing protein ActA was present in 15 different ST-specific variants, including four ActA variants with an internal truncation. In conclusion, this study shows that L. monocytogenes, isolated from illegally imported food, have moderate genotype diversity, but diverse virulence factors variants, mainly of InlA.
机构:
Inst Pasteur, French Natl Reference Ctr Listeria, Paris, France
WHO Collaborating Ctr Listeria, Biol Infect Unit, Paris, FranceInst Pasteur, French Natl Reference Ctr Listeria, Paris, France
Cantinelli, Thomas
;
Chenal-Francisque, Viviane
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机构:
Inst Pasteur, French Natl Reference Ctr Listeria, Paris, France
WHO Collaborating Ctr Listeria, Biol Infect Unit, Paris, FranceInst Pasteur, French Natl Reference Ctr Listeria, Paris, France
Chenal-Francisque, Viviane
;
Diancourt, Laure
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Inst Pasteur, Genotyping Pathogens & Publ Hlth, Paris, FranceInst Pasteur, French Natl Reference Ctr Listeria, Paris, France
机构:
Inst Pasteur, French Natl Reference Ctr Listeria, Paris, France
WHO Collaborating Ctr Listeria, Biol Infect Unit, Paris, FranceInst Pasteur, French Natl Reference Ctr Listeria, Paris, France
Leclercq, Alexandre
;
Wirth, Thierry
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Ecole Prat Hautes Etud, Lab Biol Integrat, Paris, France
CNRS, Dept Syst & Evolut, Museum Natl Hist Nat, UMR 7205, Paris, FranceInst Pasteur, French Natl Reference Ctr Listeria, Paris, France
Wirth, Thierry
;
Lecuit, Marc
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机构:
Inst Pasteur, French Natl Reference Ctr Listeria, Paris, France
WHO Collaborating Ctr Listeria, Biol Infect Unit, Paris, France
Inst Pasteur, INSERM, U1117, F-75724 Paris, France
Univ Paris 05, AP HP, Hop Univ Necker Enfants Malades, Inst Imagine,Serv Maladies Infect & Trop, Paris, FranceInst Pasteur, French Natl Reference Ctr Listeria, Paris, France
机构:
Inst Pasteur, French Natl Reference Ctr Listeria, Paris, France
WHO Collaborating Ctr Listeria, Biol Infect Unit, Paris, FranceInst Pasteur, French Natl Reference Ctr Listeria, Paris, France
Cantinelli, Thomas
;
Chenal-Francisque, Viviane
论文数: 0引用数: 0
h-index: 0
机构:
Inst Pasteur, French Natl Reference Ctr Listeria, Paris, France
WHO Collaborating Ctr Listeria, Biol Infect Unit, Paris, FranceInst Pasteur, French Natl Reference Ctr Listeria, Paris, France
Chenal-Francisque, Viviane
;
Diancourt, Laure
论文数: 0引用数: 0
h-index: 0
机构:
Inst Pasteur, Genotyping Pathogens & Publ Hlth, Paris, FranceInst Pasteur, French Natl Reference Ctr Listeria, Paris, France
机构:
Inst Pasteur, French Natl Reference Ctr Listeria, Paris, France
WHO Collaborating Ctr Listeria, Biol Infect Unit, Paris, FranceInst Pasteur, French Natl Reference Ctr Listeria, Paris, France
Leclercq, Alexandre
;
Wirth, Thierry
论文数: 0引用数: 0
h-index: 0
机构:
Ecole Prat Hautes Etud, Lab Biol Integrat, Paris, France
CNRS, Dept Syst & Evolut, Museum Natl Hist Nat, UMR 7205, Paris, FranceInst Pasteur, French Natl Reference Ctr Listeria, Paris, France
Wirth, Thierry
;
Lecuit, Marc
论文数: 0引用数: 0
h-index: 0
机构:
Inst Pasteur, French Natl Reference Ctr Listeria, Paris, France
WHO Collaborating Ctr Listeria, Biol Infect Unit, Paris, France
Inst Pasteur, INSERM, U1117, F-75724 Paris, France
Univ Paris 05, AP HP, Hop Univ Necker Enfants Malades, Inst Imagine,Serv Maladies Infect & Trop, Paris, FranceInst Pasteur, French Natl Reference Ctr Listeria, Paris, France