Culture-independent analysis of the gut microbiota in colorectal cancer and polyposis

被引:206
|
作者
Scanlan, Pauline D. [1 ,2 ]
Shanahan, Fergus [1 ,2 ,3 ]
Clune, Yvonne
Collins, John K.
O'Sullivan, Gerald C. [4 ]
O'Riordan, Micheal [5 ]
Holmes, Elaine [6 ]
Wang, Yulan [6 ]
Marchesi, Julian R. [1 ,2 ,3 ]
机构
[1] Natl Univ Ireland Univ Coll Cork, Dept Microbiol, Cork, Ireland
[2] Natl Univ Ireland Univ Coll Cork, Dept Med, Cork, Ireland
[3] Natl Univ Ireland Univ Coll Cork, Alimentary Pharmabiot Ctr, Cork, Ireland
[4] Mercy Univ Hosp, Cork Canc Res Ctr, Cork, Ireland
[5] Natl Univ Ireland Univ Coll Cork, Mercy Univ Hosp, Cork, Ireland
[6] Univ London Imperial Coll Sci Technol & Med, Dept Biomol Med, Fac Med, SORA Div, London SW7 2AZ, England
关键词
D O I
10.1111/j.1462-2920.2007.01503.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A role for the intestinal microbiota is routinely cited as a potential aetiological factor in colorectal cancer initiation and progression. As the majority of bacteria in the gut are refractory to culture we investigated this ecosystem in subjects with colorectal cancer and with adenomatous polyposis who are at high risk of developing colorectal cancer, using culture-independent methods. Twenty colorectal cancer and 20 polypectomized volunteers were chosen for this analysis. An exploration of the diversity and temporal stability of the dominant bacteria and several bacterial subgroups was undertaken using 16S rRNA gene denaturing gradient gel electrophoresis and ribosomal intergenic spacer analysis (RISA). Metabonomic analysis of the distal gut microbiota's environment was also undertaken. A significantly reduced temporal stability and increased diversity for the microbiota of subjects with colorectal cancer and polyposis was evident. A significantly increased diversity of the Clostridium leptum and C. coccoides subgroups was also noted for both disease groups. A clear division in the metabonome was observed for the colorectal cancer and polypectomized subjects compared with control volunteers. The intestinal microbiota and their metabolites are significantly altered in both colorectal cancer and polypectomized subjects compared with controls.
引用
收藏
页码:789 / 798
页数:10
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