Effects of High-Pressure Treatments (Ultra-High Hydrostatic Pressure and High-Pressure Homogenization) on Bighead Carp (Aristichthys nobilis) Myofibrillar Protein Native State and Its Hydrolysate

被引:26
作者
Chen, Mengting [1 ,2 ]
Wang, Lan [2 ]
Xie, Bijun [1 ]
Ma, Aimin [1 ]
Hu, Kai [1 ]
Zheng, Changliang [2 ]
Xiong, Guangquan [2 ]
Shi, Liu [2 ]
Ding, Anzi [2 ]
Li, Xin [2 ]
Qiao, Yu [2 ]
Sun, Zhida [1 ]
Wu, Wenjin [2 ]
机构
[1] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China
[2] Hubei Acad Agr Sci, Inst Agroprod Proc & Nucl Agr Technol, Key Lab Agr Prod Cold Chain Logist, Minist Agr & Rural Affairs, Wuhan 430064, Peoples R China
基金
国家重点研发计划;
关键词
Ultra-high hydrostatic pressure; High-pressure homogenization; Myofibrillar structure modification; Enzymatic hydrolysate; Antioxidant activity; FUNCTIONAL-PROPERTIES; ENZYMATIC-HYDROLYSIS; GELATION PROPERTIES; WHEY-PROTEIN; ANTIOXIDANT ACTIVITY; BIOACTIVE PEPTIDES; CHEMICAL FORCES; AGGREGATION; MEAT; PASTEURIZATION;
D O I
10.1007/s11947-022-02878-1
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The impacts of four treatments, ultra-high hydrostatic pressure (UHP), high-pressure homogenization (HPH), combined UHP + HPH (U-H), and HPH + UHP (H-U), on bighead carp (Aristichthys nobilis) myofibrillar protein (BMP) structure, functional hydrolysis property to pepsin, and antioxidant activity of hydrolysates were investigated. All treatments led to increase in low-molecular-weight BMP, the BMP stability, and hydrophobic group exposure, but decrease in total sulfhydryl content and BMP particle size, thus resulting in increased hydrolysis and antioxidant capacity of enzymatic hydrolysates. U-H treated BMP exhibited the highest surface hydrophobicity (924.5 +/- 1.0), zeta potential absolute value (18.88 +/- 0.11 mV), hydrolysis degree (54.5 +/- 1.6%), and antioxidant activity, but the lowest alpha-helix (21.84 +/- 2.61%), intrinsic fluorescence spectrum intensity, total sulfhydryl (7.24 +/- 0.07 mu mol/g), and mean particle size (182.57 +/- 2.23 nm). Therefore, U-H might be a promising pretreatment to prepare bioactive peptide.
引用
收藏
页码:2252 / 2266
页数:15
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