Directed evolution of (βα)8-barrel enzymes

被引：18
作者：
Höcker, B [1 ]
机构：
[1] Duke Univ, Med Ctr, Dept Biochem, Durham, NC 27710 USA
来源：
BIOMOLECULAR ENGINEERING | 2005年 / 22卷 / 1-3期
关键词：
(beta alpha)(8)-barrel; TIM-barrel; directed evolution; random mutagenesis;
D O I：
10.1016/j.bioeng.2004.09.005
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Natural molecular evolution supplies us with manifold examples of protein engineering. The imitation of these natural processes in the design of new enzymes has led to surprising and insightful results. Well-suited for design by evolutionary methods are enzymes with the common and versatile (beta alpha)(8)-barrel fold. Studies of enzyme stability, folding and design as well as the evolution of (beta alpha)(8)-barrel enzymes are discussed. (C) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：31 / 38
页数：8
相关论文
共 63 条
  • [51] Reverse engineering the (β/α)8 barrel fold
    Silverman, JA
    Balakrishnan, R
    Harbury, PB
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (06) : 3092 - 3097
  • [52] Directed evolution of Thermotoga neapolitana xylose isomerase:: high activity on glucose at low temperature and low pH
    Sriprapundh, D
    Vieille, C
    Zeikus, JG
    [J]. PROTEIN ENGINEERING, 2003, 16 (09): : 683 - 690
  • [53] SINGLE-STEP ASSEMBLY OF A GENE AND ENTIRE PLASMID FROM LARGE NUMBERS OF OLIGODEOXYRIBONUCLEOTIDES
    STEMMER, WPC
    CRAMERI, A
    HA, KD
    BRENNAN, TM
    HEYNEKER, HL
    [J]. GENE, 1995, 164 (01) : 49 - 53
  • [54] Thermophilic adaptation of proteins
    Sterner, R
    Liebl, W
    [J]. CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2001, 36 (01) : 39 - 106
  • [55] CHICKEN TRIOSEPHOSPHATE ISOMERASE COMPLEMENTS AN ESCHERICHIA-COLI DEFICIENCY
    STRAUS, D
    GILBERT, W
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (07) : 2014 - 2018
  • [56] Transforming a (β/α)8-barrel enzyme into a split-protein sensor through directed evolution
    Tafelmeyer, P
    Johnsson, N
    Johnsson, K
    [J]. CHEMISTRY & BIOLOGY, 2004, 11 (05): : 681 - 689
  • [57] Evolutionary markers in the (β/α)8-barrel fold
    Vega, MC
    Lorentzen, E
    Linden, A
    Wilmanns, M
    [J]. CURRENT OPINION IN CHEMICAL BIOLOGY, 2003, 7 (06) : 694 - 701
  • [58] Directed evolution of N-acetylneuraminic acid aldolase to catalyze enantiomeric aldol reactions
    Wada, M
    Hsu, CC
    Franke, D
    Mitchell, M
    Heine, A
    Wilson, I
    Wong, CH
    [J]. BIOORGANIC & MEDICINAL CHEMISTRY, 2003, 11 (09) : 2091 - 2098
  • [59] Structural and mutagenesis studies of leishmania triosephosphate isomerase: a point mutation can convert a mesophilic enzyme into a superstable enzyme without losing catalytic power
    Williams, JC
    Zeelen, JP
    Neubauer, G
    Vriend, G
    Backmann, J
    Michels, PAM
    Lambeir, AM
    Wierenga, RK
    [J]. PROTEIN ENGINEERING, 1999, 12 (03): : 243 - 250
  • [60] STRUCTURAL CONSERVATION IN PARALLEL BETA/ALPHA-BARREL ENZYMES THAT CATALYZE 3 SEQUENTIAL REACTIONS IN THE PATHWAY OF TRYPTOPHAN BIOSYNTHESIS
    WILMANNS, M
    HYDE, CC
    DAVIES, DR
    KIRSCHNER, K
    JANSONIUS, JN
    [J]. BIOCHEMISTRY, 1991, 30 (38) : 9161 - 9169
1234567