Comparative analysis of CreER transgenic mice for the study of brain macrophages: A case study

被引：46

作者：

Chappell-Maor, Louise ^{[1
]}

Kolesnikov, Masha ^{[1
]}

Kim, Jung-Seok ^{[1
]}

Shemer, Anat ^{[1
]}

Haimon, Zhana ^{[1
]}

Grozovski, Jonathan ^{[1
]}

Boura-Halfon, Sigalit ^{[1
]}

Masuda, Takahiro ^{[2
]}

Prinz, Marco ^{[2
,3
,4
,5
]}

Jung, Steffen ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Immunol, Rehovot, Israel

[2] Univ Freiburg, Inst Neuropathol, Fac Med, Freiburg, Germany

[3] Univ Freiburg, Ctr Basics NeuroModulat NeuroModulBasics, Fac Med, Freiburg, Germany

[4] Univ Freiburg, Signalling Res Ctr BIOSS, Freiburg, Germany

[5] Univ Freiburg, Signalling Res Ctr CIBSS, Freiburg, Germany

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 2020年 / 50卷 / 03期

基金：

欧洲研究理事会; 以色列科学基金会;

关键词：

conditional mutagenesis; CreER; fate mapping; microglia; recombination; FRACTALKINE RECEPTOR CX(3)CR1; MAMMALIAN-CELLS; RECOMBINASE; FATE; RNA; DYNAMICS; NEURONS; TISSUE;

D O I：

10.1002/eji.201948342

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Conditional mutagenesis and fate mapping have contributed considerably to our understanding of physiology and pathology. Specifically, Cre recombinase-based approaches allow the definition of cell type-specific contributions to disease development and of inter-cellular communication circuits in respective animal models. Here we compared Cx(3)cr1(CreER) and Sall1(CreER) transgenic mice and their use to decipher the brain macrophage compartment as a showcase to discuss recent technological advances. Specifically, we highlight the need to define the accuracy of Cre recombinase expression, as well as strengths and pitfalls of these particular systems that should be taken into consideration when applying these models.

引用

页码：353 / 362

页数：10

共 44 条

[1] CX3CR1+ CD8α+ dendritic cells are a steady-state population related to plasmacytoid dendritic cells [J].

Bar-On, Liat ;

Birnberg, Tal ;

Lewis, Kanako L. ;

Edelson, Brian T. ;

Bruder, Dunja ;

Hildner, Kai ;

Buer, Jan ;

Murphy, Kenneth M. ;

Reizis, Boris ;

Jung, Steffen .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (33) :14745-14750

[2] Conditional Gene-Targeting in Mice: Problems and Solutions [J].

Becher, Burkhard ;

Waisman, Ari ;

Lu, Li-Fan .

IMMUNITY, 2018, 48 (05) :835-836

[3] A Combination of Ontogeny and CNS Environment Establishes Microglial Identity [J].

Bennett, F. Chris ;

Bennett, Mariko L. ;

Yaqoob, Fazeela ;

Mulinyawe, Sara B. ;

Grant, Gerald A. ;

Gephart, Melanie Hayden ;

Plowey, Edward D. ;

Barres, Ben A. .

NEURON, 2018, 98 (06) :1170-+

[4] Sall1 is a transcriptional regulator defining microglia identity and function [J].

Buttgereit, Anne ;

Lelios, Iva ;

Yu, Xueyang ;

Vrohlings, Melissa ;

Krakoski, Natalie R. ;

Gautier, Emmanuel L. ;

Nishinakamura, Ryuichi ;

Becher, Burkhard ;

Greter, Melanie .

NATURE IMMUNOLOGY, 2016, 17 (12) :1397-1406

[5] Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition) [J].

Cossarizza, Andrea ;

Chang, Hyun-Dong ;

Radbruch, Andreas ;

Acs, Andreas ;

Adam, Dieter ;

Adam-Klages, Sabine ;

Agace, William W. ;

Aghaeepour, Nima ;

Akdis, Mubeccel ;

Allez, Matthieu ;

Almeida, Larissa Nogueira ;

Alvisi, Giorgia ;

Anderson, Graham ;

Andrae, Immanuel ;

Annunziato, Francesco ;

Anselmo, Achille ;

Bacher, Petra ;

Baldari, Cosima T. ;

Bari, Sudipto ;

Barnaba, Vincenzo ;

Barros-Martins, Joana ;

Battistini, Luca ;

Bauer, Wolfgang ;

Baumgart, Sabine ;

Baumgarth, Nicole ;

Baumjohann, Dirk ;

Baying, Bianka ;

Bebawy, Mary ;

Becher, Burkhard ;

Beisker, Wolfgang ;

Benes, Vladimir ;

Beyaert, Rudi ;

Blanco, Alfonso ;

Boardman, Dominic A. ;

Bogdan, Christian ;

Borger, Jessica G. ;

Borsellino, Giovanna ;

Boulais, Philip E. ;

Bradford, Jolene A. ;

Brenner, Dirk ;

Brinkman, Ryan R. ;

Brooks, Anna E. S. ;

Busch, Dirk H. ;

Buescher, Martin ;

Bushnell, Timothy P. ;

Calzetti, Federica ;

Cameron, Garth ;

Cammarata, Ilenia ;

Cao, Xuetao ;

Cardell, Susanna L. .

EUROPEAN JOURNAL OF IMMUNOLOGY, 2019, 49 (10) :1457-1973

[6] Single-cell profiling identifies myeloid cell subsets with distinct fates during neuroinflammation [J].

Costa Jordao, Marta Joana ;

Sankowski, Roman ;

Brendecke, Stefanie M. ;

Sagar ;

Locatelli, Giuseppe ;

Tai, Yi-Heng ;

Tay, Tuan Leng ;

Schramm, Eva ;

Armbruster, Stephan ;

Hagemeyer, Nora ;

Gross, Olaf ;

Mai, Dominic ;

Cicek, Ozgun ;

Falk, Thorsten ;

Kerschensteiner, Martin ;

Grun, Dominic ;

Prinz, Marco .

SCIENCE, 2019, 363 (6425) :365-+

[7] ATP mediates rapid microglial response to local brain injury in vivo [J].

Davalos, D ;

Grutzendler, J ;

Yang, G ;

Kim, JV ;

Zuo, Y ;

Jung, S ;

Littman, DR ;

Dustin, ML ;

Gan, WB .

NATURE NEUROSCIENCE, 2005, 8 (06) :752-758

[8] A clonogenic bone marrow progenitor specific for macrophages and dendritic cells [J].

Fogg, DK ;

Sibon, C ;

Miled, C ;

Jung, S ;

Aucouturier, P ;

Littman, DR ;

Cumano, A ;

Geissmann, F .

SCIENCE, 2006, 311 (5757) :83-87

[9]

Fonseca M. I., 2017, J NEUROINFLAMM, P1

[10]

Foo Lynette C, 2013, Cold Spring Harb Protoc, V2013, P421, DOI 10.1101/pdb.prot074211

← 1 2 3 4 5 →