Preparation, characterization and biological properties of Gellan gum films with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linker

被引：44
作者：
Lee, Ming-Wei [1 ,2 ]
Chen, Hui-Ju [1 ]
Tsao, Shu-Wei [2 ]
机构：
[1] Chung Shan Med Univ, Sch Med Lab & Biotechnol, Taichung 402, Taiwan
[2] Chung Shan Med Univ Hosp, Dept Clin Lab, Taichung 402, Taiwan
来源：
CARBOHYDRATE POLYMERS | 2010年 / 82卷 / 03期
关键词：
Gellan gum; EDC; Biocompatibility; Wound healing; IN-VITRO; BIOCOMPATIBILITY; CHITOSAN; HYDROGEL; IMPLANT; RELEASE; SKIN;
D O I：
10.1016/j.carbpol.2010.06.019
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Gellan gum (GG) is an exopolysaccharide produced by Sphingomonas elodea. To develop a medical application for GG, in this study we prepared 26 mu m thick films of GG reacted with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) in 40% ethanol to obtain a cross-linked film (GG40) with 73% gel content and 52.4 MPa tensile strength. In vitro biocompatibility tests, GG40 film exhibit nontoxic effects for L929 cells and inhibit absorption and activation of platelets. When implanted into rat subcutaneous tissue, the GG40 film caused minor inflammation in the early postoperative period. The results indicate that the effects of GG40 film on wound healing, wound size reduction (%) and collagen content are higher than those found in commercial products (Duoderm). Therefore, we conclude that the GG film developed in this study has potential for future use in surgical applications. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：920 / 926
页数：7
相关论文
共 36 条
  • [1] Controlled release of cephalexin through gellan gum beads: Effect of formulation parameters on entrapment efficiency, size, and drug release
    Agnihotri, Sunil A.
    Jawalkar, Sheetal S.
    Aminabhavi, Tejraj M.
    [J]. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2006, 63 (03) : 249 - 261
  • [2] Composite membranes based on gellan and poly(N-vinylimidazole).: Synthesis and characterization
    Alupei, IC
    Popa, M
    Bejenariu, A
    Vasiliu, S
    Alupei, V
    [J]. EUROPEAN POLYMER JOURNAL, 2006, 42 (04) : 908 - 916
  • [3] Evaluation of an in situ forming hydrogel wound dressing based on oxidized alginate and gelatin
    Balakrishnan, B
    Mohanty, M
    Umashankar, PR
    Jayakrishnan, A
    [J]. BIOMATERIALS, 2005, 26 (32) : 6335 - 6342
  • [4] BeMiller J. N., 1996, Journal of Applied Glycoscience, V43, P377
  • [5] Preparation and characterization of chitosan/poly(vinyl alcohol) chemically crosslinked blends for biomedical applications
    Costa-Junior, Ezequiel S.
    Barbosa-Stancioli, Edel F.
    Mansur, Alexandra A. P.
    Vasconcelos, Wander L.
    Mansur, Herman S.
    [J]. CARBOHYDRATE POLYMERS, 2009, 76 (03) : 472 - 481
  • [6] Biocompatibility of agarose gel as a dermal filler:: Histologic evaluation of subcutaneous implants
    Fernandez-Cossio, Sergio
    Leon-Mateos, Alvaro
    Gude Sampedro, Francisco
    Castano Oreja, Maria Teresa
    [J]. PLASTIC AND RECONSTRUCTIVE SURGERY, 2007, 120 (05) : 1161 - 1169
  • [7] Occurrence, production, and applications of gellan:: current state and perspectives
    Fialho, Arsenio M.
    Moreira, Leonilde M.
    Granja, Ana Teresa
    Popescu, Alma O.
    Hoffmann, Karen
    Sa-Correia, Isabel
    [J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2008, 79 (06) : 889 - 900
  • [8] Novel biopolymers as implant matrix for the delivery of ciprofloxacin:: Biocompatibility, degradation, and in vitro antibiotic release
    Fulzele, Suniket V.
    Satturwar, Prashant M.
    Dorle, Avinash K.
    [J]. JOURNAL OF PHARMACEUTICAL SCIENCES, 2007, 96 (01) : 132 - 144
  • [9] Polyethylene/phospholipid polymer alloy as an alternative to poly(vinylchloride)-based materials
    Ishihara, K
    Nishiuchi, D
    Watanabe, J
    Iwasaki, Y
    [J]. BIOMATERIALS, 2004, 25 (06) : 1115 - 1122
  • [10] STRUCTURAL STUDIES OF GELLAN GUM, AN EXTRACELLULAR POLYSACCHARIDE ELABORATED BY PSEUDOMONAS-ELODEA
    JANSSON, PE
    LINDBERG, B
    SANDFORD, PA
    [J]. CARBOHYDRATE RESEARCH, 1983, 124 (01) : 135 - 139
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