How low can you go? A current perspective on low-abundance proteomics

被引：17

作者：

da Costa, Joao Pinto ^{[1
,2
]}

Santos, Patricia S. M. ^{[1
,2
]}

Vitorino, Rui ^{[3
,4
]}

Rocha-Santos, Teresa ^{[1
,2
]}

Duarte, Armando C. ^{[1
,2
]}

机构：

[1] Univ Aveiro, CESAM, Campus Santiago, P-3810193 Aveiro, Portugal

[2] Univ Aveiro, Dept Chem, Campus Santiago, P-3810193 Aveiro, Portugal

[3] Univ Aveiro, Inst Biomed iBiMED, Dept Med Sci, Campus Santiago, P-3810193 Aveiro, Portugal

[4] Univ Porto, Dept Physiol & Cardiothorac Surg, Fac Med, P-4200319 Oporto, Portugal

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2017年 / 93卷

关键词：

Low-abundance; Proteomics; Depletion; Enrichment; Biomarkers; AFFINITY-CHROMATOGRAPHY; HUMAN PLASMA; PROTEIN-A; SEPARATION SYSTEMS; BIOLOGICAL-FLUIDS; CRYSTAL-STRUCTURE; LIGAND LIBRARIES; STRUCTURAL BASIS; ALBUMIN REMOVAL; CONCANAVALIN-A;

D O I：

10.1016/j.trac.2017.05.014

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The advent of proteomics brought with it the promise of protein-based diagnostics, bound to be more efficient and informative due to the intrinsic role of proteins in the molecular and cellular processes that are at the basis of both health and disease. Alas, the vast dynamic range of the proteome in biological fluids has greatly hampered the expected advances in medicine. Low-abundance proteins, which carry great diagnostic potential, are often obscured by the presence of high-abundance proteins and their identification remains technically challenging, despite the numerous developed strategies. Herein, we not only discuss the complexity of the human proteome, but also cover the most commonly used approaches for enhancing the visibility of these highly sought-after biological entities, aimed at the perhaps larger goal of this research: the discovery of biomarkers, which may lead to the long-expected personalized medicine. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：171 / 182

页数：12

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[21] Multiscale Conformational Heterogeneity in Staphylococcal Protein A: Possible Determinant of Functional Plasticity [J].