Pancreatic trypsinogen I expression during cell growth and differentiation of two human colon carcinoma cells

被引:13
作者
Bernard-Perrone, F
Carrere, J
Renaud, W
Moriscot, C
Thoreux, K
Bernard, P
Servin, A
Balas, D
Senegas-Balas, F
机构
[1] Fac Med, Grp Rech Trophicite & Vieillissement, F-06107 Nice 2, France
[2] Grp Rech Glandes Exocrines, F-13385 Marseille, France
[3] Hop Renee Sabran, F-83406 Hyeres, France
[4] Unite Format & Rech Sci Pharm, Inst Natl Sante & Rech Med 94-07, F-92296 Chatenay Malabry, France
来源
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 1998年 / 274卷 / 06期
关键词
D O I
10.1152/ajpgi.1998.274.6.G1077
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Pancreatic trypsin has been found to induce tight junction or dome formation in some colon cancer cell lines (HT-29, Caco-2), and a tumor-associated trypsinogen, trypsinogen type II, has been isolated from another colon cancer cell line (COLO 205). We have tried to determine if trypsinogen is present and how its expression varies during cell culture in KT-29 Glc+/- and Caco-2 cells, which exhibit enterocytic differentiation, and in HT-29 Glc+ cells, which never differentiate. Trypsinogen mRNA presence and expression were demonstrated in these cells by mRNA hybridization, RT-PCR, cytoimmunofluorescence, Western immunoblot analysis, and gel filtration. Trypsinogen was found to be trypsinogen type I and was mainly in zymogen form in culture media. Differentiating cells exhibited variations in trypsinogen I expression, but cells that remained undifferentiated did not. In the differentiated cells, a high and transient peak in trypsinogen I expression was observed during the first steps of differentiation.
引用
收藏
页码:G1077 / G1086
页数:10
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