Impact of different substrates on biomass protein composition during wastewater treatment investigated by two-dimensional electrophoresis

被引：5

作者：

Huber, S ^{[1
]}

Minnebusch, S ^{[1
]}

Wuertz, S ^{[1
]}

Wilderer, PA ^{[1
]}

Helmreich, B ^{[1
]}

机构：

[1] Tech Univ Munich, Inst Water Qual control & Waste Management, D-85748 Garching, Germany

来源：

WATER SCIENCE AND TECHNOLOGY | 1998年 / 37卷 / 4-5期

关键词：

activated sludge; proteins; two-dimensional gel electrophoresis (2D-PAGE); sequencing batch reactor (SBR); paper mill wastewater; microbial polymers;

D O I：

10.1016/S0273-1223(98)00131-0

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The influence of different influent substrates on biomass protein composition was examined by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Activated sludge from six sequencing batch reactors (SBRs) was investigated; four reactors were fed with model substrates and two received effluents from a wood milling process and a paper production process, respectively. Our investigations showed that in 2D-PAGE complex substrates caused a less diverse protein pattern than model wastewater composed of simple and low molecular weight compounds. This may be caused by complex formation by high molecular weight compounds of substrate with proteins, A more likely explanation is the presence of a more diversified microbial population resulting in a lower concentration of individual proteins, so that detection limits after staining were too high to observe discrete spots. (C) 1998 IAWQ. Published by Elsevier Science Ltd.

引用

页码：363 / 366

页数：4

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