MicroRNA-19a regulates the proliferation, migration and invasion of human gastric cancer cells by targeting CUL5

被引：22
作者：
Zhu, Yu [1 ]
Li, Linhai [1 ]
Hou, Dezhi [1 ]
Ouyang, Yiming [1 ]
Guo, Xiaodong [1 ]
Wang, Yongzhi [1 ]
Li, Jiyan [2 ]
Gong, Kunmei [1 ]
机构：
[1] First Peoples Hosp Yunnan Prov, Dept Gen Surg, Kunming 650032, Yunnan, Peoples R China
[2] Kunming Med Univ, Dept Pain, Affiliated Hosp 1, Kunming 650032, Yunnan, Peoples R China
来源：
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2019年 / 662卷
关键词：
microRNA; Migration; Invasion; Gastric cancer; Proliferation; CUL-5; EPIDEMIOLOGY; EXPRESSION; PREVENTION; PROTEIN; GENES;
D O I：
10.1016/j.abb.2018.11.023
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Gastric cancer is one of the prevalent types of cancers and despite improvements in its treatment, the overall survival is still far from descent. The dearth of efficient biomarkers, chemotherapeutic agents and therapeutic targets form a major hurdle in the treatment of the gastric cancer. Accumulating evidences suggest that MicroRNAs (miRs) may prove important therapeutic targets/agents for the management of cancers including gastric cancer. Herein, we examined the expression of miR-19a by qRT-PCR in gastric cancer and attempted to explore its potential role. It was found that the expression of miR-19a is significantly (p < 0.05) enhanced in the gastric cancer tissues as well as the gastric cancer cell lines. Inhibition of miR-19a in gastric cancer cells suppressed the proliferation migration and invasion of the gastric cancer cells. Bioinformatic analysis revealed CUL5 to be the potential target of miR-19a. Contrary, to the expression of miR-19a, the expression of CUL5 was significantly (p < 0.05) downregulated in all the gastric cancer tissues and cell lines. However, inhibition of miR-19a in SNU-16 gastric cancer cells could cause upsurge of CUL5 expression. Overexpression of CUL-5 was found to exhibit similar effects on the proliferation, migration and invasion of the SNU-16 gastric cancer cells as that of miR-19a suppression. Additionally, overexpression of CUL5 could at least partially abolish the effects of miR-19a suppression on the proliferation, migration and invasion of SNU-16 gastric cancer cells. Finally, overexpression of miR-19a caused inhibition of the xenografted tumors in vivo indicating the potential of miR-19a as therapeutic target for gastric cancer.
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页码：93 / 100
页数：8
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