Hydrophilic interaction chromatography of intact, soluble proteins

被引：51

作者：

Tetaz, Tim ^{[1
]}

Detzner, Simon ^{[1
]}

Friedlein, Arno ^{[2
]}

Molitor, Birgit ^{[3
]}

Mary, Jean-Luc ^{[3
]}

机构：

[1] F Hoffmann La Roche Ltd, Discovery Technol, PROBMX21, CH-4070 Basel, Switzerland

[2] F Hoffmann La Roche Ltd, Translat Res Sci, PRLP, CH-4070 Basel, Switzerland

[3] F Hoffmann La Roche Ltd, Metab & Vasc Res, PRDM, CH-4070 Basel, Switzerland

来源：

JOURNAL OF CHROMATOGRAPHY A | 2011年 / 1218卷 / 35期

关键词：

Hydrophilic Interaction Chromatography; Lipophilic proteins; Apolipoproteins; PolyHYDROXYETHYL A; ZIC-HILIC PEEK; ProntoSIL; TSKgel Amide 80; PERFORMANCE LIQUID-CHROMATOGRAPHY; APOLIPOPROTEIN-A-IV; SEPARATION; PEPTIDES; ACIDS;

D O I：

10.1016/j.chroma.2010.09.027

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The separation of intact proteins by means of Hydrophilic Interaction Chromatography (HILIC) was demonstrated with human apoA-I, recombinant human apoM, and equine cytochrome C. Five different commercially available HILIC columns were compared. Using one of these columns, different glycosylated isoforms of apoM were separated from each other and from the aglyco-form. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：5892 / 5896

页数：5

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