Synaptic Vesicles Studied by SAXS: Derivation and Validation of a Model Form Factor

被引：11

作者：

Castorph, S. ^{[1
]}

Arleth, L. ^{[2
]}

Sztucki, M. ^{[3
]}

Vainio, U. ^{[4
]}

Ghosh, S. K. ^{[1
]}

Holt, M. ^{[5
]}

Jahn, R. ^{[5
]}

Salditt, T. ^{[1
]}

机构：

[1] Georg August Univ Gottingen, Inst Rontgenphys, Gottingen, Germany

[2] Univ Copenhagen, Fac Life Sci, Biophys, Frederiksberg, Denmark

[3] European Synchrotron Radiat Fac, Grenoble, France

[4] DESY, Hamburger Synchrotronstrahlungslab, Hamburg, Germany

[5] Max Planck Inst Biophys Chem, Dept Neurol, Gottingen, Germany

来源：

XIV INTERNATIONAL CONFERENCE ON SMALL-ANGLE SCATTERING (SAS09) | 2010年 / 247卷

关键词：

SMALL-ANGLE SCATTERING; BLOCK-COPOLYMER MICELLES; X-RAY-SCATTERING; BEAMLINE;

D O I：

10.1088/1742-6596/247/1/012015

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

We discuss different spherically symmetric and anisotropic form factor models and test them against high resolution synchrotron based small-angle x-ray scattering (SAXS) data from synaptic vesicles (SVs), isolated from rat brain. Anisotropy of the model form factors is found to be a key ingredient for the description of the native synaptic vesicle structure. We describe changes in structural parameters due to protease digestion of SVs, and present SAXS data of SVs recorded under different pH conditions.

引用

页数：17

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