Induction of neural stem cell-like cells (NSCLCs) from mouse astrocytes by Bmi1

被引：37

作者：

Moon, Jai-Hee ^{[1
,2
]}

Yoon, Byung Sun ^{[1
,2
]}

Kim, Bona ^{[1
,2
]}

Park, Gyuman ^{[3
]}

Jung, Hye-Youn ^{[1
]}

Maeng, Isaac ^{[1
]}

Jun, Eun Kyoung ^{[1
,2
]}

Yoo, Seung Jun ^{[1
]}

Kim, Aeree ^{[4
]}

Oh, Sejong ^{[5
]}

Whang, Kwang Youn ^{[1
]}

Kim, Hyunggee ^{[1
]}

Kim, Dong-Wook ^{[6
]}

Kim, Ki Dong ^{[2
]}

You, Seungkwon ^{[1
]}

机构：

[1] Korea Univ, Coll Life Sci & Biotechnol, Lab Cell Growth & Funct Regulat, Seoul 136701, South Korea

[2] Imgen Co Ltd, Suwon, Gyenggi Do, South Korea

[3] Korea Univ, Coll Med, Dept Dermatol, Seoul 136705, South Korea

[4] Korea Univ, Coll Med, Dept Pathol, Seoul 136705, South Korea

[5] Chonnam Natl Univ, Inst Agr Sci & Technol, Dept Anim Sci, Kwangju, South Korea

[6] Yonsei Univ, Coll Med, Ctr Cell Therapy, Dept Physiol, Seoul, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 371卷 / 02期

关键词：

neural stem cells; astrocytes; dedifferentiation; neural stem cell-like cells;

D O I：

10.1016/j.bbrc.2008.04.068

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently, Bmi1 was shown to control the proliferation and self-renewal of neural stem cells (NSCs). In this study, we demonstrated the induction of NSC-like cells (NSCLCs) from mouse astrocytes by Bmi1 under NSC culture conditions. These NSCLCs exhibited the morphology and growth properties of NSCs, and expressed NSC marker genes, including nestin, CD133, and Sox2. In vitro differentiation of NSCLCs resulted in differentiated cell populations containing astrocytes, neurons, and oligodendrocytes. Following treatment with histone deacetylase inhibitors (trichostatin A and valproic acid), the potential of NSCLCs for proliferation, dedifferentiation, and self-renewal was significantly inhibited. Our data indicate that multipotent NSCLCs can be generated directly from astrocytes by the addition of Bmi1. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：267 / 272

页数：6

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