Oligoethylenimines Grafted to PEGylated Poly(β-amino ester)s for Gene Delivery

被引：21

作者：

Xia, Jialiang ^{[1
,2
]}

Tian, Huayu ^{[1
]}

Chen, Lei ^{[1
,2
]}

Lin, Lin ^{[1
]}

Guo, Zhaopei ^{[1
]}

Chen, Jie ^{[1
]}

Chen, Xuesi ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, Key Lab Polymer Ecomat, Changchun 130022, Peoples R China

[2] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

来源：

BIOMACROMOLECULES | 2011年 / 12卷 / 04期

基金：

中国国家自然科学基金;

关键词：

LOW-MOLECULAR-WEIGHT; IN-VITRO; POLYETHYLENIMINE; TRANSFECTION; VECTORS; DIACRYLATE; COPOLYMERS; POLYMERS; CHITOSAN; CARRIERS;

D O I：

10.1021/bm101361g

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Novel polymers composed of net-like PEGylated poly(beta-amino ester) (N-P-1, M-w = 6900 or N-P-2, M-w = 21 400) and oligoethylenimine (OEI) (OEI423 or OEI600) were synthesized and evaluated as gene carriers. The molecular weights of these polymers were well-controlled by the concentration of the cross-linking reaction. The synthesized polymers showed high biodegradability, less cytotoxicity, and efficient DNA retard ability. The N-P-1-OEI600/DNA complex showed much slower aggregation in the presence of 10 and 20% serum solutions. In vitro transfection assays, N-P-2-OEI423, N-P-1-OEI600, and N-P-2-OEI600 showed enhanced transfection efficiency compared with the PEI25K control in the presence or in the absence of serum in different cell lines. In particular, in Cos-7 cells, the transfection efficiency of N-P-1-OEI600 was 20.9 times higher than that of PEI25K in the presence of serum. The polymer/DNA complex stability, lower cytotoxicity, and higher transfection efficiency in the presence of serum revealed that N-P-1-OEI600 could be a potential nonviral gene carrier for In Vivo application.:

引用

页码：1024 / 1031

页数：8

共 39 条

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