Single-step purification of different lipases from Staphylococcus warneri

被引：26

作者：

Volpato, Giandra ^{[1
,2
]}

Filice, Marco ^{[1
]}

Ayub, Marco A. Z. ^{[2
]}

Guisan, Jose M. ^{[1
]}

Palomo, Jose M. ^{[1
]}

机构：

[1] CSIC, Inst Catalisis, Dept Biocatalisis, Madrid, Spain

[2] Univ Fed Rio Grande do Sul, Inst Food Sci & Technol, Porto Alegre, RS, Brazil

来源：

JOURNAL OF CHROMATOGRAPHY A | 2010年 / 1217卷 / 04期

关键词：

Interfacial activation; Purification; Interaction lipase-lipase; Staphylococcus warneri; HYDROPHOBIC SUPPORTS; TRIACYLGLYCEROL LIPASE; INTERFACIAL ACTIVATION; SELECTIVE ADSORPTION; IMMOBILIZATION; PROTEINS; HYPERACTIVATION; STABILIZATION; INHIBITOR; MECHANISM;

D O I：

10.1016/j.chroma.2009.11.055

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Three different lipases from the extract crude of Staphylococcus warneri have been purified by specific lipase-lipase interactions using different lipases (TLL, RML, PFL, BTL2) covalently attached to a solid support as adsorption matrix. BTL2 immobilized on glyoxyl-DTT adsorbed selectivity only a 30 kDa lipase from the crude, which was desorbed by adding 0.1% triton X-100. Using glyoxyl-PFL as matrix, two new lipases (28 and 40 kDa) were adsorbed, and completely pure 40 kDa lipase was obtained after desorption using 0.01% triton, whereas 28 kDa lipase was desorbed after the incubation of the lipase matrix with 3% detergent. When using other matrixes as glyoxyl-TLL or glyoxyl-RML, different lipases were adsorbed. This methodology could be a very efficient and useful method to purify several lipases from crude extracts from different sources. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：473 / 478

页数：6

共 36 条

[1] HYDROLYSIS OF EDIBLE OILS BY LIPASES IMMOBILIZED ON HYDROPHOBIC SUPPORTS - EFFECTS OF INTERNAL SUPPORT STRUCTURE [J].

ALDURI, B ;

ROBINSON, E ;

MCNERLAN, S ;

BAILIE, P .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1995, 72 (11) :1351-1359

[2] ENZYMATIC-SYNTHESIS OF FATTY ESTERS BY HYDROPHOBIC LIPASE DERIVATIVES IMMOBILIZED ON ORGANIC POLYMER BEADS [J].

BASRI, M ;

AMPON, K ;

YUNUS, WMZW ;

RAZAK, CNA ;

SALLEH, AB .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1995, 72 (04) :407-411

[3]

Bastida A, 1998, BIOTECHNOL BIOENG, V58, P486, DOI 10.1002/(SICI)1097-0290(19980605)58:5<486::AID-BIT4>3.0.CO

[4]

2-9

[5] Chemoenzymatic dynamic kinetic resolution of allylic alcohols:: A highly enantioselective route to acyloin acetates [J].

Bogar, Krisztian ;

Vidal, Pilar Hoyos ;

Leon, Andres R. Alcantara ;

Backvall, Jan-E. .

ORGANIC LETTERS, 2007, 9 (17) :3401-3404

[6] The presence of thiolated compounds allows the immobilization of enzymes on glyoxyl agarose at mild pH values: New strategies of stabilization by multipoint covalent attachment [J].

Bolivar, Juan M. ;

Lopez-Gallego, Fernando ;

Godoy, Cesar ;

Rodrigues, Dasciana S. ;

Rodrigues, Rafael C. ;

Batalla, Pilar ;

Rocha-Martin, Javier ;

Mateo, Cesar ;

Giordano, Raquel L. C. ;

Guisan, Jose M. .

ENZYME AND MICROBIAL TECHNOLOGY, 2009, 45 (6-7) :477-483

[7] A SERINE PROTEASE TRIAD FORMS THE CATALYTIC CENTER OF A TRIACYLGLYCEROL LIPASE [J].

BRADY, L ;

BRZOZOWSKI, AM ;

DEREWENDA, ZS ;

DODSON, E ;

DODSON, G ;

TOLLEY, S ;

TURKENBURG, JP ;

CHRISTIANSEN, L ;

HUGEJENSEN, B ;

NORSKOV, L ;

THIM, L ;

MENGE, U .

NATURE, 1990, 343 (6260) :767-770

[8] A MODEL FOR INTERFACIAL ACTIVATION IN LIPASES FROM THE STRUCTURE OF A FUNGAL LIPASE-INHIBITOR COMPLEX [J].

BRZOZOWSKI, AM ;

DEREWENDA, U ;

DEREWENDA, ZS ;

DODSON, GG ;

LAWSON, DM ;

TURKENBURG, JP ;

BJORKLING, F ;

HUGEJENSEN, B ;

PATKAR, SA ;

THIM, L .

NATURE, 1991, 351 (6326) :491-494

[9] Activation of Bacterial Thermoalkalophilic Lipases Is Spurred by Dramatic Structural Rearrangements [J].

Carrasco-Lopez, Cesar ;

Godoy, Cesar ;

de las Rivas, Blanca ;

Fernandez-Lorente, Gloria ;

Palomo, Jose M. ;

Guisan, Jose M. ;

Fernandez-Lafuente, Roberto ;

Martinez-Ripoll, Martin ;

Hermoso, Juan A. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (07) :4365-4372

[10] CATALYSIS AT THE INTERFACE - THE ANATOMY OF A CONFORMATIONAL CHANGE IN A TRIGLYCERIDE LIPASE [J].

DEREWENDA, U ;

BRZOZOWSKI, AM ;

LAWSON, DM ;

DEREWENDA, ZS .

BIOCHEMISTRY, 1992, 31 (05) :1532-1541

← 1 2 3 4 →