A consensus list of microsatellite markers for olive genotyping

被引：160

作者：

Baldoni, Luciana ^{[1
]}

Cultrera, Nicolo G. ^{[1
]}

Mariotti, Roberto ^{[1
]}

Ricciolini, Claudia ^{[1
]}

Arcioni, Sergio ^{[1
]}

Vendramin, Giovanni G. ^{[2
]}

Buonamici, Anna ^{[2
]}

Porceddu, Andrea ^{[3
]}

Sarri, Vania ^{[4
]}

Ojeda, Maria A. ^{[5
]}

Trujillo, Isabel ^{[5
]}

Rallo, Luis ^{[5
]}

Belaj, Angjelina ^{[6
]}

Perri, Enzo ^{[7
]}

Salimonti, Amelia ^{[7
]}

Muzzalupo, Innocenzo ^{[7
,8
]}

Casagrande, Alberto ^{[9
]}

Lain, Orietta ^{[10
]}

Messina, Rachele ^{[10
]}

Testolin, Raffaele ^{[9
,10
]}

机构：

[1] CNR, Ist Genet Vegetale, I-06128 Perugia, Italy

[2] CNR, Ist Genet Vegetale, I-50019 Florence, Italy

[3] Univ Sassari, DSGVA, I-07100 Sassari, Italy

[4] Univ Perugia, Dipartimento Biol Cellulare & Ambientale, Sez Biol Cellulare & Mol, I-06100 Perugia, Italy

[5] Univ Cordoba, Dept Agron, E-14080 Cordoba, Spain

[6] IFAPA, Ctr Alameda Obispo, Cordoba 14083, Spain

[7] Ctr Ric Olivicoltura & Ind, CRA, I-87036 Arcavacata Di Rende, CS, Italy

[8] Ctr Ric Prod Foraggere & Lattiero Casearie, CRA, I-26900 Lodi, Italy

[9] Ist Genom Applicata, I-33100 Udine, Italy

[10] Univ Udine, Dipartimento Sci Agr & Ambientali, I-33100 Udine, Italy

来源：

MOLECULAR BREEDING | 2009年 / 24卷 / 03期

关键词：

SSR; Fingerprinting; Cultivar characterization; Olea europaea; Repeat motif; Allelic ladder; OLEA-EUROPAEA L; SEQUENCE REPEATS SSRS; GENETIC DIVERSITY; CULTIVAR IDENTIFICATION; DNA; SET; AFLP; VARIETIES; GERMPLASM; SELECTION;

D O I：

10.1007/s11032-009-9285-8

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Cultivar identification is a primary concern for olive growers, breeders, and scientists. This study was aimed at examining the SSR markers retrieved from the literature and currently used in olive study, in order to select those most effective in characterizing the olive accessions and to make possible the comparison of data obtained by different laboratories. Olive microsatellite profiles were assessed by four independent laboratories, which analyzed 37 pre-selected SSR loci on a set of 21 cultivars. These SSR markers were initially tested for their reproducibility, power of discrimination and number of amplified loci/alleles. Independent segregation was tested for each pair of SSRs in a controlled cross and the allelic error rate was quantified. Some of them were finally selected as the most informative and reliable. Most of the alleles were sequenced and their sizes were determined. Profiles of the reference cultivars and a list of alleles with their sizes obtained by sequencing are reported. Several genetic parameters have been analysed on a larger set of cultivars allowing for a deeper characterization of the selected loci. Results of this study provide a list of recommended markers and protocols for olive genotyping as well as the allelic profile of a set of reference cultivars that would be useful for the establishment of a universal database of olive accessions.

引用

页码：213 / 231

页数：19

共 65 条

[1] Long perfect dinucleotide repeats are typical of vertebrates, show motif preferences and size convergence [J].