Efficient Enzymatic Process for Mulberry Paper Production: An Approach for Xylooligosaccharide Production Coupled with Minimizing Bleaching Agent Doses

This study focused on the applying endo-xylanases to reduce the use of bleaching agent, coupled with the production of xylooligosaccharides (XOs), in mulberry paper making. Paper mulberry pulp (PMP) was consecutively prepared from paper mulberry bark by traditional NaOH-treatment, and two types of thermostable endo-xylanase from Streptomyces thermovulgaris TISTR1948 (wild-type and recombinant endo-xylanases) were employed in the biobleaching of PMP. This process was optimized to achieve maximum XOs yields and the highest PMP quality. The optimal condition was an enzyme dosage of 125 U/g PMP at 12 h of reaction time, both in a 500 mL laboratory bottle and a 150 L reactor. The mixture obtained from the reactor was separated as liquid of XOs derived from PMP (PMP-XOs) and solid biobleached PMP. The PMP-XOs from wild-type endo-xylanase were composed of 31.6% xylopentaose (X5), 30.9% xylohexaose and higher-degree XOs (X >= 6), and 11.7% xylobiose (X2), whereas 76.6% of X5 and 8.6% of X2 were the main products from recombinant endo-xylanase. The PMP-XOs derived from both endo-xylanase types exhibited high antioxidant activities, reducing power, phenolic contents, and prebiotic efficacy. In addition, the application of both endo-xylanases enhanced the brightness of PMP by 5.1% and 3.5%, and reduced the kappa number by 9.1% and 3.6%, respectively. Biobleached PMP was subsequently subjected to the NaOCl bleaching step to produce the mulberry paper. This approach could reduce NaOCl consumption by 20-25%, making it an environmentally friendly alternative. The production of valuable prebiotics, such as PMP-XOs, further enhances the economic viability of this approach.