LcERF2 modulates cell wall metabolism by directly targeting a UDP-glucose-4-epimerase gene to regulate pedicel development and fruit abscission of litchi

被引:24
|
作者
Yi, Jun-Wen [1 ]
Wang, Yi [1 ]
Ma, Xiao-Sha [1 ]
Zhang, Jie-Qiong [2 ]
Zhao, Ming-Lei [1 ]
Huang, Xu-Ming [1 ]
Li, Jian-Guo [1 ]
Hu, Gui-Bing [1 ]
Wang, Hui-Cong [1 ,2 ]
机构
[1] South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Key Lab Biol & Genet Improvement Hort Crops South, Guangdong Litchi Engn Res Ctr,Coll Hort, Guangzhou, Peoples R China
[2] Yangtze Normal Univ, Dept Life Sci & Technol, Fuling 408100, Peoples R China
来源
PLANT JOURNAL | 2021年 / 106卷 / 03期
关键词
APETALA2/ethylene-response factor; cell wall metabolism; fruit abscission; UDP-glucose-4-epimerase gene; Litchi chinensis; pedicel development; FLORAL ORGAN ABSCISSION; TRANSCRIPTION FACTOR; ETHYLENE BIOSYNTHESIS; LIGNIN BIOSYNTHESIS; ERF GENES; ARABIDOPSIS; EXPRESSION; ZONE; DEGRADATION; POLYGALACTURONASES;
D O I
10.1111/tpj.15201
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Elucidating the biochemical and molecular basis of premature abscission in fruit crops should help develop strategies to enhance fruit set and yield. Here, we report that LcERF2 contributes to differential abscission rates and responses to ethylene in Litchi chinensis (litchi). Reduced LcERF2 expression in litchi was observed to reduce fruit abscission, concurrent with enhanced pedicel growth and increased levels of hexoses, particularly galactose, as well as pectin abundance in the cell wall. Ecoptic expression of LcERF2 in Arabidopsis thaliana caused enhanced petal abscission, together with retarded plant growth and reduced pedicel galactose and pectin contents. Transcriptome analysis indicated that LcERF2 modulates the expression of genes involved in cell wall modification. Yeast one-hybrid, dual-luciferase reporter and electrophoretic mobility shift assays all demonstrated that a UDP-glucose-4-epimerase gene (LcUGE) was the direct downstream target of LcERF2. This result was further supported by a significant reduction in the expression of the A. thaliana homolog AtUGE2-4 in response to LcERF2 overexpression. Significantly reduced pedicel diameter and enhanced litchi fruit abscission were observed in response to LcUGE silencing. We conclude that LcERF2 mediates fruit abscission by orchestrating cell wall metabolism, and thus pedicel growth, in part by repressing the expression of LcUGE.
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页码:801 / 816
页数:16
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