Cloning, expression, purification, crystallization and preliminary crystallographic analysis of pseudo death-effector domain of HIPPI, a molecular partner of Huntingtin-interacting protein HIP-1

被引：1

作者：

Banerjee, Manisha ^{[1
]}

Majumder, Pritha ^{[1
]}

Bhattacharyya, Nitai P. ^{[1
]}

Dattagupta, Jiban K. ^{[1
]}

Sen, Udayaditya ^{[1
]}

机构：

[1] Saha Inst Nucl Phys, Struct Genom Sect, Kolkata 700064, W Bengal, India

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2006年 / 62卷

关键词：

APOPTOSIS; DEDD;

D O I：

10.1107/S1744309106046628

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The formation of a heterodimer between Huntingtin-interacting protein-1 (HIP-1) and its novel partner HIPPI (HIP-1 protein interactor) through their pseudo death-effector domains (pDEDs) is a key step that recruits caspase-8 and initiates apoptosis. This could be one of the pathways by which apoptosis is increased in Huntington's disease (HD). A construct consisting of the pDED of HIPPI has been cloned and overexpressed as 6NH-tagged protein and purified by Ni-NTA affinity chromatography. Crystals of the pDED of HIPPI were grown in space group P4(1), with unit-cell parameters a = b = 77.42, c = 33.31 angstrom and a calculated Matthews coefficient of 1.88 angstrom(3) Da(-1) (33% solvent content) with two molecules per asymmetric unit.

引用

页码：1247 / 1250

页数：4

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