Highly sensitive GQDs-MnO2 based assay with turn-on fluorescence for monitoring cerebrospinal acetylcholinesterase fluctuation: A biomarker for organophosphorus pesticides poisoning and management

被引：41

作者：

Deng, Jingjing ^{[1
]}

Lu, Dingkun ^{[1
]}

Zhang, Xiaolei ^{[1
]}

Shi, Guoyue ^{[2
]}

Zhou, Tianshu ^{[1
]}

机构：

[1] East China Normal Univ, Sch Ecol & Environm Sci, 500 Dongchuan Rd, Shanghai 200241, Peoples R China

[2] East China Normal Univ, Sch Chem & Mol Engn, 500 Dongchuan Rd, Shanghai 200241, Peoples R China

来源：

ENVIRONMENTAL POLLUTION | 2017年 / 224卷

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

Turn-on fluorescent assay; GQDs-MnO2; Cerebrospinal acetylcholinesterase; Organophosphorus pesticides; QUANTUM DOTS; TEMPERATURE; NANOSHEET; SENSOR; PROBE; DNA; NANOPARTICLES; INSECTICIDES; PRALIDOXIME; EXPOSURE;

D O I：

10.1016/j.envpol.2017.02.024

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

In this study, we demonstrated an assay with turn-on fluorescence for monitoring cerebrospinal acetylcholinesterase (AChE) fluctuation as a biomarker for organophosphorus pesticides (OPs) poisoning and management based on single layer MnO2 nanosheets with graphene quantum dots (GQDs) as signal readout. Initially, the fluorescence of GQDs was quenched by MnO2 nanosheets mainly due to the inner filter effect (IFE). However, with the presence of reductive thiocholine (TCh), the enzymatic product, hydrolyzed from acetylthiocholine (ATCh) by AChE, the redox reaction between MnO2 and TCh occurred, leading to the destruction of the MnO2 nanosheets, and thereby IFE was diminished gradually. As a consequence, the turn-on fluorescence of GQDs with the changes in the spectrum of the dispersion constituted a new mechanism for sensing of cerebrospinal AChE. With the method developed here, we could monitor cerebrospinal AChE fluctuation of rats exposed to OPs before and after therapy, and could thereby open up the pathway to a new sensing platform for better understanding the mechanism of brain dysfunctions associate with OPs poisoning. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：436 / 444

页数：9

共 51 条

[1] Persistence of selected organophosphate and carbamate insecticides in waters from a coastal watershed [J].