Lipid Nanotube Encapsulating Method in Low-Energy Scanning Transmission Electron Microscopy Analyses

被引：12

作者：

Furusho, Hirotoshi ^{[1
]}

Mishima, Yumiko ^{[2
]}

Kameta, Naohiro ^{[3
,5
]}

Yamane, Midori ^{[4
]}

Masuda, Mitsutoshi ^{[3
,5
]}

Asakawa, Masumi ^{[3
,5
]}

Yamashita, Ichiro ^{[4
]}

Mori, Hirotaro ^{[1
]}

Takaoka, Akio ^{[1
]}

Shimizu, Toshimi ^{[3
,5
]}

机构：

[1] Osaka Univ, Res Ctr Ultra High Voltage Electron Microscopy, Osaka 5670047, Japan

[2] CNRS, Ctr Biophys Mol, F-45071 Orleans, France

[3] Japan Sci & Technol Agcy, SORST, Tsukuba, Ibaraki 3058565, Japan

[4] Nara Inst Sci & Technol, Grad Sch Mat Sci, Nara 6300192, Japan

[5] Natl Inst Adv Ind Sci & Technol, Nanotube Res Ctr NTRC, Tsukuba, Ibaraki 3058565, Japan

来源：

JAPANESE JOURNAL OF APPLIED PHYSICS | 2009年 / 48卷 / 09期

关键词：

MOLECULES;

D O I：

10.1143/JJAP.48.097001

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

The lipid nanotube (LNT) encapsulating method is a rational sample fixation method that can be used to mount samples for transmission electron microscopy analyses. By employing the LNT encapsulating method in 30 kV low-voltage scanning transmission electron microscopy (LV-STEM), it is possible to record multiangle images of ferritin without using the negative staining method. We have also recorded a tilted series of high-contrast LV-STEM images and reconstructed three-dimensional images. These results show that LNTs have sufficient durability for LV electron beam, and indicate the potential of the LNT encapsulating method as a sample fixation method of LV electron microscopy. (C) 2009 The Japan Society of Applied Physics

引用

页码：0970011 / 0970015

页数：5

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