Peroxynitrite induces the conversion of xanthine dehydrogenase to oxidase in rabbit liver

被引：49

作者：

Sakuma, S

Fujimoto, Y

Sakamoto, Y

Uchiyama, T

Yoshioka, K

Nishida, H

Fujita, T

机构：

[1] Department of Hygienic Chemistry, Osaka Univ. of Pharmaceutical Sci., Takatsuki, Osaka 569-11, 4-20-1, Nasahara

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 230卷 / 02期

关键词：

D O I：

10.1006/bbrc.1996.5983

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Effect of peroxynitrite (ONOO-) on the conversion of xanthine dehydrogenase to oxidase in rabbit liver was examined. ONOO- (25-200 mu M) induced the conversion of xanthine dehydrogenase to oxidase in a dose-dependent manner. The addition of hydroxyl radical scavengers (mannitol and dimethyl sulfoxide) gave no alteration in the ONOO--induced conversion of xanthine dehydrogenase to oxidase, implying that the action of ONOO- is not due to hydroxyl radicals which may be formed from ONOO-. The experiment utilizing dithiothreitol also revealed that the action of ONOO- might be due to oxidation of sulfhydryl group of xanthine dehydrogenase. These results suggest that ONOO- has the potential to convert xanthine dehydrogenase to oxidase, and that this effect may be correlated with cytotoxic actions of ONOO-. (C) 1997 Academic Press

引用

页码：476 / 479

页数：4

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