The marine pathogen Vibrio vulnificus encodes a putative homologue of the Vibrio harveyi regulatory gene, luxR:: a genetic and phylogenetic comparison

被引:45
作者
McDougald, D
Rice, SA
Kjellberg, S [1 ]
机构
[1] Univ New S Wales, Sch Microbiol & Immunol, Sydney, NSW 2052, Australia
[2] Univ New S Wales, Ctr Marine Biofouling & Bioinnovat, Sydney, NSW 2052, Australia
基金
澳大利亚研究理事会;
关键词
molecular evolution; quorum sensing; signaling genes; transcriptional regulator; virulence;
D O I
10.1016/S0378-1119(00)00117-7
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Vibrio vulnificus is an opportunistic pathogen that exhibits numerous virulence factors, including the secretion of a zinc metalloprotease and the production of a capsule. We have cloned and sequenced a gene from V. vulnificus that is a homologue of the positive transcriptional regulator, luxR, of the lux operon in Vibrio harveyi. This gene encodes a putative, single complete open reading frame designated smcR, which shares greater than 75% nucleotide identity with luxR of V. harveyi. The deduced amino acid sequence of the putative SmcR protein is more than 90% identical and 95% similar to that of LuxR of V. harveyi, suggesting that V. vulnificus possesses a member of the family of signal-response genes recently described in Vibrio cholerae and in Vibrio parahaemolyticus . Our data also demonstrate that, in addition to V. vulnificus, all six Vibrio spp. tested contained genes that hybridized with the luxR probe. We also present evidence that this regulatory protein was inherited from a common ancestor, and that the gene is ancient and widespread in marine Vibrio spp. (C) 2000 Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:213 / 221
页数:9
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