A cDNA clone for β-caryophyllene synthase from Artemisia annua

被引:117
作者
Cai, Y
Jia, JW
Crock, J
Lin, ZX
Chen, XY
Croteau, R [1 ]
机构
[1] Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA
[2] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Lab Plant Mol Genet, Shanghai 200032, Peoples R China
[3] Shanghai Jiao Tong Univ, Coll Life Sci & Biotechnol, Shanghai 200030, Peoples R China
关键词
Artemisia annua; Asteraceae; sweet wormwood; homology-based cDNA cloning; recombinant protein expression; sesquiterpene synthase; beta-caryophyllene synthase; sesquiterpene; beta-caryophyllene; gene expression; elicitation;
D O I
10.1016/S0031-9422(02)00265-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An homology-based cloning strategy yielded a full-length cDNA from Artemisia annua that encoded a protein of 60.3 kDa which resembled a sesquiterpene synthase in sequence. Heterologous expression of the gene in Escherichia coli provided a soluble recombinant enzyme capable of catalyzing the divalent metal ion-dependent conversion of farnesyl diphosphate to beta-caryophyllene, a sesquiterpene olefin found in the essential oil of A. annua. In reaction parameters and kinetic properties, beta-caryophyllene synthase resembles other sesquiterpene synthases of angiosperms. The beta-caryophyllene synthase gene is expressed in most plant tissues during early development, and is induced in mature tissue in response to fungal elicitor thus suggesting a role for beta-caryophyllene in plant defense. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:523 / 529
页数:7
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