Comparison of different extraction methods for polysaccharides from Dendrobium officinale stem

被引：192
作者：
He, Lian [1 ]
Yan, Xiaoteng [4 ]
Liang, Jian [2 ,3 ]
Li, Shijie [2 ,3 ,5 ]
He, Hongrui [5 ]
Xiong, Qingping [2 ,3 ,5 ]
Lai, Xiaoping [2 ,3 ,6 ,7 ]
Hou, Shaozhen [2 ,3 ,6 ,7 ]
Huang, Song [2 ,3 ,6 ,7 ]
机构：
[1] Guangdong Food & Drug Vocat Coll, Guangzhou 510520, Guangdong, Peoples R China
[2] Guangzhou Univ Chinese Med, Sch Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China
[3] Guangzhou Univ Chinese Med, Guangdong Prov Key Lab New Drug Dev & Res Chinese, Guangzhou 510006, Guangdong, Peoples R China
[4] Xuzhou Med Univ, Affiliated Huaian Hosp, Huaian 223001, Jiangsu, Peoples R China
[5] Jiangsu Key Lab Reg Resource Exploitat & Med Res, Huaian 223003, Jiangsu, Peoples R China
[6] Dongguan Math Engn Acad Chinese Med, Dongguan 523808, Guangdong, Peoples R China
[7] Guangzhou Univ Chinese Med, Dongguan 523808, Guangdong, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2018年 / 198卷
基金：
中国国家自然科学基金;
关键词：
Dendrobium officinale stem polysaccharide; Extraction method; Yield; Characteristic; Antioxidant activity; SCANNING-ELECTRON-MICROSCOPY; ANTIOXIDANT ACTIVITY; OXIDATIVE STRESS; PURIFIED POLYSACCHARIDE; ABTS; INFLAMMATION; PRESSURE;
D O I：
10.1016/j.carbpol.2018.06.073
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The purpose of this study was to screen the optimum extraction of polysaccharides (DOP) from Dendrobium officinale stem. Firstly, different methods, including hot water extraction (HWE), cold-pressing (CP), freeze-thawing cold-pressing (FTCP), ultrasonic-assisted hot water extraction (UHWE), microwave-assisted hot water extraction (MHWE) and enzyme-assisted hot water extraction (EHWE), were employed to extract DOP under their respective best parameters. Then, the extraction yield, structure and antioxidant activity of the polysaccharides from different extraction methods were compared under the same condition. The data implied that UHWE and FTCP possessed higher extraction yield than the other extraction methods. Besides, DOPCP and DOPFTCP had higher molecular weight than the other polysaccharide samples. More importantly, DOPFTCP had the highest antioxidant activity. Overall, DOPFTCP exhibit high extraction yield, well-preserved molecular chains and best antioxidant activity, all these indicated FTCP was the most suitable method to extract DOP.
引用
收藏
页码：101 / 108
页数：8
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