Molecularly Imprinted Polymer (MIP) Film with Improved Surface Area Developed by Using Metal-Organic Framework (MOF) for Sensitive Lipocalin (NGAL) Determination

被引:70
作者
Iskierko, Zofia [1 ]
Sharma, Piyush Sindhu [1 ]
Prochowicz, Daniel [1 ]
Fronc, Krzysztof [2 ]
D'Souza, Francis [3 ]
Toczydlowska, Diana [4 ]
Stefaniak, Filip [4 ]
Noworyta, Krzysztof [1 ]
机构
[1] Polish Acad Sci, Inst Phys Chem, Kasprzaka 44-52, PL-01224 Warsaw, Poland
[2] Polish Acad Sci, Inst Phys, 32-46 Al Lotnikow, PL-02668 Warsaw, Poland
[3] Univ North Texas, Dept Chem, Denton, TX 76203 USA
[4] Int Inst Mol & Cell Biol Warsaw, Lab Bioinformat & Prot Engn, Ks Trojdena 4, PL-02109 Warsaw, Poland
基金
欧洲研究理事会;
关键词
NGAL; human neutrophil gelatinase-associated lipocalin; field effect transistor; EG-FET; molecularly imprinted polymer; metal-organic framework; molecular docking; PROTEINS; GROWTH;
D O I
10.1021/acsami.6b05515
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Electropolymerizable functional and cross-linking monomers were used to prepare conducting molecularly imprinted polymer film with improved surface area with the help of a sacrificial metal organic framework (MOF). Subsequent dissolution of the MOF layer resulted in a surface developed MIP film. This surface enlargement increased the analyte accessibility to imprinted molecular cavities. Application of the porous MIP film as a recognition unit of an extended-gate field effect transistor (EG-FET) chemosensor effectively enhanced analytical current signals of determination of recombinant human neutrophil gelatinase-associated lipocalin (NGAL)
引用
收藏
页码:19860 / 19865
页数:6
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