MEF2D-BCL9 Fusion Gene Is Associated With High-Risk Acute B-Cell Precursor Lymphoblastic Leukemia in Adolescents

被引：104

作者：

Suzuki, Kyogo ^{[1
]}

Okuno, Yusuke ^{[1
,2
]}

Kawashima, Nozomu ^{[1
]}

Muramatsu, Hideki ^{[1
]}

Okuno, Tatsuya ^{[1
]}

Wang, Xinan ^{[1
]}

Kataoka, Shinsuke ^{[1
]}

Sekiya, Yuko ^{[1
]}

Hamada, Motoharu ^{[1
]}

Murakami, Norihiro ^{[1
]}

Kojima, Daiei ^{[1
]}

Narita, Kotaro ^{[3
]}

Narita, Atsushi ^{[1
]}

Sakaguchi, Hirotoshi ^{[3
]}

Sakaguchi, Kimiyoshi ^{[4
]}

Yoshida, Nao ^{[3
]}

Nishio, Nobuhiro ^{[1
,2
]}

Hama, Asahito ^{[1
]}

Takahashi, Yoshiyuki ^{[1
]}

Kudo, Kazuko ^{[5
]}

Kato, Koji ^{[3
]}

Kojima, Seiji ^{[1
]}

机构：

[1] Nagoya Univ, Sch Med, Nagoya, Aichi, Japan

[2] Nagoya Univ Hosp, Nagoya, Aichi, Japan

[3] Japanese Red Cross Nagoya First Hosp, Childrens Med Ctr, Nagoya, Aichi, Japan

[4] Hamamatsu Univ Sch Med, Hamamatsu, Shizuoka, Japan

[5] Fujita Hlth Univ, Sch Med, Toyoake, Aichi, Japan

来源：

JOURNAL OF CLINICAL ONCOLOGY | 2016年 / 34卷 / 28期

关键词：

MEF2D/DAZAP1; EXPRESSION; DISCOVERY; APOPTOSIS; SURVIVAL; CLONING; KINASE; CANCER; HDAC9;

D O I：

10.1200/JCO.2016.66.5547

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Purpose Acute lymphoblastic leukemia (ALL) makes up a significant proportion of all pediatric cancers, and relapsed ALL is a leading cause of cancer-associated deaths in children. Identification of risk factors and druggable molecular targets in ALL can lead to a better stratification of treatments and subsequent improvement in prognosis. Patients and Methods We enrolled 59 children with relapsed or primary refractory ALL who were treated in our institutions. We primarily performed RNA sequencing (RNA-seq) using patients' leukemic cells to comprehensively detect gene fusions and analyze gene expression profiles. On the basis of results obtained by RNA-seq, we performed genetic validation, functional analysis, and in vitro drug sensitivity testing using patients' samples and an exogenous expression model. Results We identified a total of 26 gene fusions in 22 patients by RNA-seq. Among these, 19 were nonrandom gene fusions already described in ALL, and four of the remaining seven involved identical combination of MEF2D and BCL9. All MEF2D-BCL9-positive patients had B-cell precursor immunophenotype and were characterized as being older in age, being resistant to chemotherapy, having very early relapse, and having leukemic blasts that mimic morphologically mature B-cell leukemia with markedly high expression of HDAC9. Exogenous expression of MEF2D-BCL9 in a B-cell precursor ALL cell line promoted cell growth, increased HDAC9 expression, and induced resistance to dexamethasone. Using a primary culture of leukemic blasts from a patient, we identified several molecular targeted drugs that conferred inhibitory effects in vitro. Conclusion A novel MEF2D-BCL9 fusion we identified characterizes a novel subset of pediatric ALL, predicts poor prognosis, and may be a candidate for novel molecular targeting. (C) 2016 by American Society of Clinical Oncology

引用

页码：3451 / +

页数：11

共 35 条

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