Differential activity of lytic polysaccharide monooxygenases on celluloses of different crystallinity. Effectiveness in the sustainable production of cellulose nanofibrils

被引：41

作者：

Valenzuela, Susana V. ^{[1
,2
]}

Valls, Cristina ^{[3
]}

Schink, Viviane ^{[1
]}

Sanchez, Daniel ^{[1
]}

Blanca Roncero, M. ^{[3
]}

Diaz, Pilar ^{[1
,2
]}

Martinez, Josefina ^{[1
,2
]}

Javier Pastor, F. I. ^{[1
,2
]}

机构：

[1] Univ Barcelona, Fac Biol, Dept Genet Microbiol & Stat, Ave Diagonal 643, E-08028 Barcelona, Spain

[2] Univ Barcelona, Inst Nanosci & Nanotechnol IN2UB, Barcelona, Spain

[3] Univ Politecn Cataluna, Barcelona Tech, CELBIOTECH Paper Engn Res Grp, Terrassa 08222, Spain

来源：

CARBOHYDRATE POLYMERS | 2019年 / 207卷

关键词：

Crystallinity; SamLPMO10C; Monooxygenase; Cellulose; Nanofibrils; LPMO; NFC; ENZYMATIC-HYDROLYSIS; OXIDATION; CONVERSION; BINDING; FIBERS; ACID; WOOD;

D O I：

10.1016/j.carbpol.2018.11.076

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A series of cellulosic substrates has been produced, treated with lytic polysaccharide monooxygenase (LPMO) from Streptomyces ambofaciens (SamLPMO10C), and analyzed by high performance anion exchange chromatography (HPAEC) with pulsed amperometric detection (PAD). The activity of the bacterial LPMO showed high variability depending on the origin and degree of crystallinity of the substrate. Additionally, we tested the effectiveness of SamLPMO10C in the nanofibrillation of flax, a high crystalline agricultural fiber, as a single pretreatment or in combination with cellulases. All pretreatments were followed by a mechanical defibrillation by high-pressure homogenization (HPH) to obtain cellulose nanofibrils (NFC). The combined LPMO-cellulase treatment showed higher fibrillation yield, optical transmittance and carboxylate content than control reactions. Therefore, it could be explored as a promising green alternative to reduce the energy consumption in the production of NFC. To our knowledge, this is the first study reporting the effect of a bacterial LPMO in nanocellulose production.

引用

页码：59 / 67

页数：9

共 47 条

[21] The effect of hemicelluloses on wood pulp nanofibrillation and nanofiber network characteristics [J].