Rapid and simultaneous visual typing of high-risk HPV-16/18 with use of integrated lateral flow strip platform

被引：7

作者：

Chen, Qi ^{[1
,2
]}

Yao, Li ^{[1
,2
]}

Wu, Qian ^{[1
,2
]}

Xu, Jianguo ^{[1
,2
]}

Yan, Chao ^{[1
,2
]}

Guo, Chuanxiang ^{[3
]}

Zhang, Chao ^{[3
]}

Xu, Tao ^{[4
]}

Qin, Panzhu ^{[1
,2
,4
]}

Chen, Wei ^{[1
,2
,5
]}

机构：

[1] Hefei Univ Technol, Engn Res Ctr Bioproc, MOE, Hefei 230009, Peoples R China

[2] Hefei Univ Technol, Sch Food & Biol Engn, Hefei 230009, Peoples R China

[3] Anhui Deepblue Med Technol Co Ltd, Hefei 230088, Peoples R China

[4] Anhui Med Univ, Hefei 230009, Peoples R China

[5] Hefei Univ Technol, Intelligent Mfg Inst, Hefei 230009, Peoples R China

来源：

MICROCHIMICA ACTA | 2022年 / 189卷 / 09期

基金：

中国博士后科学基金;

关键词：

Molecular amplification; Rapid screening; Visualized sensing; Nucleic acid lateral flow strip; Human papillomavirus; Two-cancer screening; HPV GENOTYPES; PCR; MICROARRAY; VARIANTS; CHIP;

D O I：

10.1007/s00604-022-05449-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A biosensor for rapid and simultaneous visual identification of high-risk human papillomavirus (HPV) genotypes 16 and 18 in clinical samples based on polymerase chain reaction (PCR) integrated lateral flow strip platform was developed. Using an one-step protocol to extract nucleic acid rapidly and the functionalized primer sets specific to HPV-16 and 18 were designed for the simultaneous amplification. In the presence of target HPV genotypes, the corresponding functionalized primer sets will participate in the PCR process and produce numerous duplex functionalized dsDNA amplicons. With the bridge effect of duplex functionalized dsDNA amplicons between gold nanoparticles-fluorescein isothiocyanate antibody conjugates (AuNP-FITC antibody conjugates) and other two antibodies on corresponding test line (T-1 or T-2), visualized color signals on test lines could be obtained directly visible with a naked eye. Combining the high amplification efficiency of PCR and the visualized sensing of LFS, as low as 700 copies of HPV-16 and 18 DNA were detected simultaneously within 75 min, which can promote application in the resource limited settings.

引用

页数：11

共 44 条

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