A new species of Osteocephalus (Osteocephalus duellmani)is described from the Ecuadorian part of the Cordillera del Condor, an isolated sub-Andean mountain range along the Ecuadorian-Peruvian border. Apparently an endemic to these mountains, it is one of the few species in the genus reaching an altitude of almost 2000 m. It is distinguished from other species in the O. buckleyi complex in the lack of spinous dorsal tuberculation, a relatively small tympanum, uniformly coloured posterior surfaces of the thighs, lack of a row of tubercles on the lower jaw, and smooth fringes on the inner side of finger III and outer edge of finger IV.