Actinobacillus succinogenes sp. nov., a novel succinic-acid-producing strain from the bovine rumen

Strain 130Z(T) was isolated from the bovine rumen, It is a facultatively anaerobic, pleomorphic, Gram-negative rod. It exhibits a 'Morse code' form of morphology, which is characteristic of the genus Actinobacillus. Strain 130Z(T) is a capnophilic, osmotolerant succinogen that utilizes a broad range of sugars. It accumulates high concentrations of succinic acid (> 70 g l(-1)). Strain 130Z(T) is positive for catalase, oxidase, alkaline phosphatase and P-galactosidase, but does not produce indole or urease, Acid but no gas is produced from D-glucose and D-fructose, 16S rRNA sequence analysis places strain 130Z(T) within the family Pasteurellaceae; the most closely related members of the family Pasteurellaceae have 16S rRNA similarities of 95.5 % or less with strain 130Z(T). Strain 130Z(T) was compared with Actinobacillus lignieresii and the related Bisgaard Taxa 6 and 10, Based upon morphological and biochemical properties, strain 130Z(T) is most similar to members of the genus Actinobacillus within the family Pasteurellaceae. It is proposed that strain 130Z(T) be classified as a new species, Actinobacillus succinogenes. The type strain of Actinobacillus succinogenes sp, nov. is ATCC 55618(T).